运用关联度分析硒镧复合作用下巴西蘑菇氨基酸含量与砷含量的关系

通过采用混合培养料试验、日立8810型氨基酸自动分析仪和原子吸收法测定氨基酸含量和砷含量以及灰色系统理论分析关联度,研究了硒镧复合作用下巴西蘑菇子实体氨基酸含量和砷含量的变化和关系,为栽培砷含量低的巴西蘑菇提供科学依据.各硒镧复合处理的关联度排序为:B4>B1>B2>B0>B3.其中B4处理关联度最大,为0.835 1,B2最小,为0.726 9,说明硒镧浓度对巴西蘑菇子实体各氨基酸含量与砷含量有一定影响.各氨基酸含量与重金属砷含量关联度顺序为:丙氨酸>酪氨酸>胱氨酸>苯丙氨酸>赖氨酸>组氨酸>天门冬氨酸>异亮氨酸>苏氨酸>缬草氨酸>亮氨酸>甘氨酸>脯氨酸>精氨酸>丝氨酸>谷氨酸>甲硫氨酸.其中丙氨酸含量与砷含量关联度最大,为0.856 6,说明硒镧复合作用下巴西蘑菇子实体中丙氨酸含量与重金属砷含量的关系最为密切;关联度最小的是甲硫氨酸.

Relational Grade Analysis on Relationship of Amino Acid Contents and Arsenic Content in Agaricus brasiliensis Under Interaction of Selenium and Lanthanium

The mixed material culture experiment was conducted,and the amino acid and arsenic(As) contents were measured by Hitachi 8810 Automatic Amino acid Analyzer and Atomic Absorption Spectrometry(AAS).Then the relationship of amino acid contents and As content in the fruiting bodies of Agaricus brasiliensis under the interaction of selenium(Se) and lanthanium(La) was analyzed by grey system theory in order to provide a scientific basis for application of Se and La with reasonable amounts to produce mushroom with low As content.The order of relational grade of the treatments was B4 > B1 > B2 > B0> B3,among which,B4 obtained the highest relational grade with a value of 0.835 1,while B2 got the lowest grade with a value of 0.726 9.It indicated that the concentrations of Se and La had certain effects on As and amino acid contents in the fruiting bodies of A.brasiliensis.Under the interaction of Se and La,the order of relational grade of the 17 amino acids with As content was Ala > Tyr > Cys > Phe > Lys > His > Asp > Ile > Thr > Val > Leu > Gly > Pro > Arg > Ser > Glu > Met.The content of Ala had the highest relational grade with As content among the 17 amino acids with a value of 0.856 6,followed by Tyr and Cys and the lowest was Met.