| CO厌氧发酵制氢过程中的微生物特性及其影响因素 |
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| 引用本文： | 赵亚,刘志军,刘凤霞,GUIOT Serge R. CO厌氧发酵制氢过程中的微生物特性及其影响因素[J]. 应用与环境生物学报, 2012, 0(4): 656-660 |
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| 作者姓名： | 赵亚 刘志军 刘凤霞 GUIOT Serge R |
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| 作者单位： | 大连理工大学化工与环境生命学部;加拿大科学研究院生物技术研究所 |
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| 基金项目： | 加拿大科学研究院-中国教育部联合培养博士生奖学金项目(No.2008NMFP)资助~~ |
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| 摘 要： | 以发酵液中溶解的一氧化碳(CO)为底物,研究高温嗜热菌(Carboxydothermus hydrogenoformans)厌氧发酵制氢的工艺过程.通过C.hydrogenoformans菌的生长规律、絮凝能力和反应特性等实验研究,建立菌株的生长规律模型,得出微生物衰减系数和最大比生长速率.结果表明,C.hydrogenoformans菌产氢率高,絮凝效果好,用于连续CO生物发酵制氢工艺是可行的.对发酵制氢过程的影响因素进行考察,得出最佳食微比及CO对发酵制氢过程的抑制浓度等过程参数,为有效开发CO厌氧生物发酵制氢的工艺路线提供了参考依据.图4表2参17
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| 关 键 词： | 生物制氢 厌氧发酵 嗜热微生物 一氧化碳 底物抑制 |
Microbial Characteristics and Influence Factors During Anaerobic Fermentation for Biohydrogen Production from CO |
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| Affiliation: | ZHAO Ya1,2,LIU Zhijun1**,LIU Fengxia1& GUIOT Serge R2(1Faculty of Chemical & Environmental & Biological Science and Technology,Dalian University of Technology,Dalian 116024,China)(2Biotechnology Research Institute,National Research Council of Canada,Montreal H4P 2R2,Canada) |
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| Abstract: | The biohydrogen production from anaerobic fermentation was studied with thermophilic bacterium Carboxydothermus hydrogenoformans using carbon monoxide(CO) dissolved in fermentation broth as the substrate.Bacterium growth model,decay efficient and the maximum specific growth rate were observed based on the experimental study of biomass growing,flocculation and microbial characteristics.The results indicated the feasibility of continuous anaerobic fermentation for hydrogen production from CO with C.hydrogenoformans due to its good hydrogen yield and flocculation ability.Furthermore,by investigating the effect of procedure parameters during CO fermentation,the optimal feed/microorganism was proposed and the maximum CO concentration was observed to avoid CO inhibition which was the key factor to consider in the study of continuous biohydrogen production from CO fermentation.Fig 4,Tab 2,Ref 17 |
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| Keywords: | biohydrogen production anaerobic fermentation thermophilic bacterium CO substrate inhibition |
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