葡萄糖和L-丙氨酸对藻菌共生系统处理养殖废水的影响研究

利用莱茵衣藻(Chlamydomonas reinhardtii CC-125)与凝结芽孢杆菌(Bacillus coagulans BS32)构建藻菌共生系统,通过Biolog EcoPlate^TM微孔板筛选系统可利用碳源,进而研究葡萄糖和L-丙氨酸对藻菌共生系统中微藻和细菌生长的影响,并考察其对养殖废水的净化能力。结果表明,藻菌共生系统对葡萄糖衍生物类碳源具有偏好性。添加0.5 g/L葡萄糖和100μmol/L L-丙氨酸可以提高藻菌共生系统对养殖废水的处理效果,连续培养5 d后养殖废水中COD、氨氮、TN、TP去除率分别从63.21%、60.68%、58.58%、80.49%提高到72.47%、91.07%、96.65%和89.29%。在藻菌共生系统中,葡萄糖可以促进莱茵衣藻和凝结芽孢杆菌生物量的增加,微量的L-丙氨酸可作为凝结芽孢杆菌的萌发剂,刺激芽孢杆菌的萌发,加速藻菌共生系统对养殖废水中有机物的利用。

The effect of glucose and L-alanine in the microalgal-bacterial consortia on culture wastewater treatment

A microalgal-bacterial consortia was constructed using Chlamydomonas reinhardtii CC-125 and Bacillus coagulans BS32.The available carbon sources of consortia were screened through Biolog EcoPlate^TM microplates,the effect of glucose and L-alanine on the growth of microalgae and bacteria was investigated,and the performance of microalgal-bacterial consortia for culture wastewater purification was examined.The results showed that the microalgal-bacterial consortia had preferences for glucose derivative carbon source,adding proper amount of glucose(0.5 g/L)and L-alanine(100μmol/L)could improve the treatment efficient of microalgal-bacterial consortia,removal rates of COD,ammonia nitrogen,TN and TP in culture wastewater increased from 63.21%,60.68%,58.58%,80.49%to 72.47%,91.07%,96.65%and 89.29%respectively after 5 days of cultivation.In microalgal-bacterial consortia,glucose could increase the biomass of Chlamydomonas reinhardtii CC-125 and Bacillus coagulans BS32,trace L-alanine as the germination regent of Bacillus coagulans BS32 could promote its bourgeon,and accelerate the utilization of organic matter in culture wastewater.