| DNA碱解旋速率法及其在DNA损伤研究中的应用 |
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| 引用本文： | 孟紫强,张连珍. DNA碱解旋速率法及其在DNA损伤研究中的应用[J]. 环境科学学报, 1988, 8(1): 56-60 |
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| 作者姓名： | 孟紫强 张连珍 |
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| 作者单位： | 山西大学环境保护系(孟紫强)，核工业部第七所(张连珍) |
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| 摘 要： | 对DNA碱解旋速率法的细胞DNA经碱解旋后通过羟基磷灰石(HA)柱分离单、双链DNA时的洗脱次数、温度以及DNA回收效果进行了研究。结果表明,用0.125mol/L和0.25mol/L磷酸钾缓冲液各3ml,先后各洗脱一次,即可满足细胞DNA链断裂研究的要求。洗脱双链DNA时的温度为60℃即可。~3H-DNA与细胞其它组分一起通过HA柱时,~3H-DNA的回收率达94％左右。应用这一技术对电离辐射引起的中国田鼠肺细胞和小鼠脾细胞DNA单链断裂损伤与照射剂量的关系进行了探讨。结果表明,DNA单链断裂程度与照射剂量呈正相关。
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| 关 键 词： | DNA解旋法 DNA链断裂 电离辐射 中国田鼠肺细胞 小鼠脾细胞 |
| 收稿时间： | 1987-02-09 |
A METHOD FOR DETERMINATION OF STRAND SEPARATION RATE OF DNA IN ALKALI AND ITS USE INDNA DAMAGE STUDY |
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| Meng Ziqiang and Zhang Lianzheng. A METHOD FOR DETERMINATION OF STRAND SEPARATION RATE OF DNA IN ALKALI AND ITS USE INDNA DAMAGE STUDY[J]. Acta Scientiae Circumstantiae, 1988, 8(1): 56-60 |
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| Authors: | Meng Ziqiang and Zhang Lianzheng |
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| Affiliation: | Department of Environmental Sciences,Shanxi University,Taiyuan and Institute for Radiation Protection,MNI |
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| Abstract: | A method for determination of strand separation rate of mammalian cell DNA in alkali medium was investigated and used to study the single-strand break of DNA in cultured Chinese hamster lung (CHL) cells and mouse splenocytes induced by 60Co y-ray. It was shown that the percentage of labelled 3H-DNA remained double strand after 30 min alkali treatment was inversely proportional to the radiation dose within the range of 1-10 Gy. |
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| Keywords: | DNA unwinding technique DNA strand break CHL cell mouse splenocyte ionizing radiation. |
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