| 环境雌激素的生物检测与应用 |
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| 引用本文： | 张静云,吕剑,何义亮,靳强.环境雌激素的生物检测与应用[J].环境科学与技术,2007,30(2):100-102. |
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| 作者姓名： | 张静云 吕剑 何义亮 靳强 |
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| 作者单位： | 上海交通大学环境科学与工程学院,上海 200240 |
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| 摘 要： | 环境雌激素问题已成为环境领域的研究热点。因此,建立简单快速有效的检测方法显得尤其重要。目前对污染物雌激素效应的测评主要依赖于生物学方法,文章主要介绍了子宫生长实验、肝细胞卵黄蛋白原生成实验、人乳腺癌细胞(MCF-7)增值实验、重组基因酵母实验、酵母双杂交实验五种主要的环境雌激素生物检测方法及其在国内外环境激素测评中的应用现状。
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| 关 键 词： | 环境雌激素 子宫生长 肝细胞卵黄蛋白 MCF-7 重组基因酵母 酵母双杂交 |
| 文章编号： | 1003-6504（2007）02-0100-03 |
| 修稿时间： | 2006-03-09 |
Bioassay and Application of Environmental Estrogens |
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| ZHANG Jing-yun,LV Jian,HE Yi-liang,JIN Qiang.Bioassay and Application of Environmental Estrogens[J].Environmental Science and Technology,2007,30(2):100-102. |
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| Authors: | ZHANG Jing-yun LV Jian HE Yi-liang JIN Qiang |
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| Abstract: | Establishment of effective and rapid assay of environmental estrogens is in urge need. Five effective and commonly used bioassay methods including uterine growth assay, vitellogenin of liver cells assay, MCF-7 cells assay, recombinant yeast assay and yeast two-hybrid assay were reviewed, and their applications in recent research on estrogens in environment were also discussed. |
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| Keywords: | environmental estrogens uterine growth vitellogenin of liver cells MCF-7 cells recombinant yeast assay yeast two-hybrid assay |
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