| 高效产絮凝剂Pseudomonas alcaligenes培养条件优化及应用研究 |
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| 引用本文： | 王远红,董滨,毛艳丽,闫永胜. 高效产絮凝剂Pseudomonas alcaligenes培养条件优化及应用研究[J]. 环境科学与技术, 2010, 33(3). DOI: 10.3969/j.issn.1003-6504.2010.03.016 |
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| 作者姓名： | 王远红 董滨 毛艳丽 闫永胜 |
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| 作者单位： | 1. 河南城建学院环境与市政工程系,河南,平顶山,467001
2. 江苏大学化学化工学院,江苏,镇江,212013 |
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| 基金项目： | 河南省科技厅科技攻关项目，上海市科委重大项目，科技部十一五科技支撑项目 |
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| 摘 要： | 以筛选出的一株高效絮凝剂产生菌产碱假单胞菌PS-25为试验菌种,采用单因素试验方法确定最佳碳源为葡萄糖,最佳氮源为黄豆饼粉。采用正交试验设计方法,对该菌产絮凝剂的培养条件进行优化研究,得到最佳培养条件是:碳源为葡萄糖(20.0g/L)、氮源为黄豆饼粉(4.0g/L)、培养基初始pH值为6.5、培养温度为30℃、接种量5%(V/V)、通气量为160r/min。在最佳培养条件下,PS-25产生的絮凝剂对高岭土悬浊液絮凝率达95.77%,且对多种实际废水都有较好的净化效果,其中对浊度的去除率均在91%以上,对色度的去除率均在80%以上,对废水中COD的去除率为54.25%～90.33%。
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| 关 键 词： | 微生物絮凝剂 产碱假单胞菌PS-25 正交试验 培养条件优化 废水处理 |
Bioflocculant-producing Pseudomonas alcaligenes:Optimal Culture and Application |
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| WANG Yuan-hong,DONG Bin,MAO Yan-li,YAN Yong-sheng. Bioflocculant-producing Pseudomonas alcaligenes:Optimal Culture and Application[J]. Environmental Science and Technology, 2010, 33(3). DOI: 10.3969/j.issn.1003-6504.2010.03.016 |
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| Authors: | WANG Yuan-hong DONG Bin MAO Yan-li YAN Yong-sheng |
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| Affiliation: | WANG Yuan-hong1,DONG Bin2,MAO Yan-li1,YAN Yong-sheng3 (1. Department of Municipal Engineering,Henan University of Urban Construction,Pingdingshan 467001,China,2. School of Environmental Science and Engineering,Tongji University,Shanghai 200092,3. School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China) |
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| Abstract: | A strain named Pseudomonas alcaligenes PS-25 was screened, with which an experimental study was conducted to determine the optimized culture condition, carbon and nitrogen sources favorable for the strain production being glucose and soybean flour respectively. The result of relevant orthogonal test indicated the optimal condition was initial pH 6.5, tem-perature 30℃, inoculum size (V/V) 5%, and shaking speed 160ffmin. Flocculating efficiency achieved for Kaolin suspension was as high as 95.77%, and fairly high removals of turbidity, color and COD from wastewater were showed as well. |
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| Keywords: | biofloccuant Pseudomonas alcaligenes orthogonai test culture condition optimization wastewater treatment |
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