| 固定CO2基因工程菌的构建 |
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| 引用本文： | 王竞,周集体,杨凤林,王栋,项学敏,HYEOB Roh Jung. 固定CO2基因工程菌的构建[J]. 应用与环境生物学报, 1999, 5(5): 512-514 |
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| 作者姓名： | 王竞 周集体 杨凤林 王栋 项学敏 HYEOB Roh Jung |
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| 作者单位： | 1. 大连理工大学环境科学与工程系,大连,116012
2. 日本地球环境产业技术研究机构 |
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| 摘 要： | 构建了含有ＲｕｂｉｓＣＯＦｏｒｍ ＩＩ基因的可转化大肠杆菌紫色非硫杆菌穿梭质粒（ＰＭＰＢ２），将其转化紫色非硫杆菌野生型Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓｐａｌｕｓｔｒｉｓ Ｎｏ．７ 和紫色非硫杆菌ＲｕｂｉｓＣＯＦｏｒｍ Ｉ缺陷型ＲｈｏｄｏｐｓｅｕｄｏｍｏｎａｓｐａｌｕｓｔｒｉｓＮｏ．７ＤＦＩ，获得２ 株ＲｕｂｉｓＣＯ基因工程菌ＭＧ１１ 和ＭＧ１４，二者的ＲｕｂｉｓＣＯ 酶活性比对照菌分别提高７６ ．７％ 和８３．３％ ．连续传４０ 代表明，重组质粒在受体菌中是较稳定的
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| 关 键 词： | RubisCO 沼泽红假单胞菌 工程菌 构建 |
CONSTRUCTION OF THE GENE ENGINEERING STRAINS FOR CO2 |
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| WANG Jing,ZHOU Jiti,YANG Fenglin,WANG Dong,XIANG Xuemin,HYEOB Roh Jung. CONSTRUCTION OF THE GENE ENGINEERING STRAINS FOR CO2[J]. Chinese Journal of Applied and Environmental Biology, 1999, 5(5): 512-514 |
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| Authors: | WANG Jing ZHOU Jiti YANG Fenglin WANG Dong XIANG Xuemin HYEOB Roh Jung |
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| Abstract: | An E. coli Rhodopseudomonas palustris shuttle plasmid,PMPB 2 containing FormII RubisCO gene was constructed, and transformed to Rhodopseudomonas palustris No.7 and No.7 RubisCO FormI deficient, respectively.The results indicated that the RubisCO enzyme activity of the engineering strains,MG11 and MG14 increased by 76.7%and 83.3% compared with wild strains. After 40 generations, the RubisCO enzyme activity of MG11 and MG14 were kept at 152 U/g and 160 U/g, respectively. |
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| Keywords: | Rhodopseudomonas palustris rubisCO engineering strains |
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