高效石油烃降解菌的筛选及其对原油污染土壤的修复

从陕北原油污染土壤中筛选出7株高效石油烃降解菌,其中黄杆菌属CC-2、不动细菌属SC-5、假单胞菌属SC-6表现出较强的石油烃降解能力。通过单因素试验和正交试验考察总石油烃(TPH)降解效果的影响因素,得出各因素对TPH降解率影响程度的大小次序为:溶液p H降解温度降解菌接种量摇床转速,且在降解菌接种量为7%(φ)、溶液p H为7、降解温度为30℃、摇床转速为150 r/min的最适处理条件下,菌株SC-6的TPH降解率可达61.23%。原油污染土壤生物修复实验结果表明:高效石油烃降解菌的投加有利于土壤TPH降解率和酶活性的提高;"菌株SC-6+营养剂"组修复处理42 d后的TPH降解率可达57.59%。

Screening of high-efficiency petroleum hydrocarbon degrading strains and remediation of crude oil contaminated soil

The 7 strains of high-efficiency petroleum hydrocarbon degrading bacteria were screened from crude oil contaminated soil in Northern Shaanxi. Among them,the strain CC-2 was Flavobacteriaceae sp.,SC-5 was Acinetobacter sp. and SC-6 was Pseudomonas sp.,showing a strong ability of petroleum hydrocarbon degradation. The factors affecting total petroleum hydrocarbon（TPH）degradation were investigated by a single factor test and orthogonal experiment,with the results showing that the order of influence was as follows：solution pH>degradation temperature>degradation bacteria inoculation amount> shaking speed. Under the optimum processing conditions of degradation bacteria inoculation amount 7%（φ）,solution pH 7,degradation temperature 30 ℃ and shaking speed 150 r/min,the TPH degradation rate by strain SC-6 reached 61.23%. The experimental results of bioremediation of crude oil contaminated soil showed that：The addition of high-efficiency petroleum hydrocarbon degrading bacteria was beneficial to the improvement of soil TPH degradation rate and enzyme activity;The TPH degradation rate of the “strain SC-6 + nutrient” group was 57.59% after 42 days’ treatment.