| 微囊藻毒素-LR完全抗原的设计及制备 |
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| 引用本文： | 盛建武,何苗,宋保栋,施汉昌,钱易. 微囊藻毒素-LR完全抗原的设计及制备[J]. 环境科学, 2005, 26(3): 33-37 |
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| 作者姓名： | 盛建武 何苗 宋保栋 施汉昌 钱易 |
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| 作者单位： | 清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084 |
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| 基金项目： | 国家高技术研究发展计划(863)项目(2002AA649160) |
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| 摘 要： | 从偶联位点、偶联剂、载体蛋白和偶联步骤等分析出发,设计了微囊藻毒素-LR (Microcystin-LR ,MC-LR)完全抗原的制备方法.在半抗原分子第7位氨基酸分子上引进1个自由的氨基;再用戊二醛2步法将此中间产物(H2N-etMC-LR)分别与BSA和OVA偶联.中间产物和偶联产物分别经固相萃取和透析纯化后,经SDS凝胶电泳、紫外扫描及生物质谱技术鉴定,结果表明MC-LR与BSA的平均偶联比能达到5以上,满足了进一步免疫的要求.
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| 关 键 词： | 微囊藻毒素-LR 完全抗原 偶联比 |
| 文章编号： | 0250-3301(2005)03-0033-05 |
| 收稿时间： | 2004-09-06 |
| 修稿时间： | 2004-10-15 |
Synthesis of Complete Antigen of Microcystin-LR and Its Antibody Production |
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| SHENG Jian-wu,HE Miao,SONG Bao-dong,SHI Han-chang and QIAN Yi. Synthesis of Complete Antigen of Microcystin-LR and Its Antibody Production[J]. Chinese Journal of Environmental Science, 2005, 26(3): 33-37 |
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| Authors: | SHENG Jian-wu HE Miao SONG Bao-dong SHI Han-chang QIAN Yi |
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| Affiliation: | ESPC State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China. |
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| Abstract: | Based on the analysis of coupling location, coupling regent, carrying protein and coupling process, completed antigen of Microcystin-LR (MC-LR) was synthesized. A free amidogen was introduced to microcystin-LR (MC-LR) using chemical modification (aminoethylation) of its 7th core amino acids, N-methyl-dehydroalanine. The intermediate product Aminoethyl-MC-LR (H2N-etMC-LR) was purified by Solid Phase Elute (SPE) and identified by Mass Spectrometry,then coupled with BSA through glutaraldehyde to be complete antigen. The completed antigen was dialyzed and identified by SDS-PAGE,UV-scanning and bio-mass spectrometry. The result show that the coupling ratio between MC-LR and BSA of complete antigen in average was over 5, and it is sufficient to immunity. |
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| Keywords: | Microcystin-LR complete antigen coupling ratio |
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