Untersuchungskonzept zur Charakterisierung toxischer Stoffe in belastetem Wasser

Simple biotests are frequently used for the ecotoxicological assessment of waste water. Unfortunately, and not too rarely, effects are detected and the question concerning water-polluting substances has to be answered. The latter are enriched by solid phase extraction (SPE), then isolated and fractioned by thinlayer chromatography using automatic multiple development (AMD). One strip of the DC-plate is separated and used without further atterations for the biological detection with microorganisms (Bacillus subtilis, luminous bacteria). With this system, toxic bands can be identified. From the other part of the DC plate, an UV-spectrum can be taken of the toxic bands using a DC scanner. Subsequently, the corresponding bands are scratched out and the toxic substance is eluted and analysed with infrared spectroscopy. The infrared spectrum can be characterised by comparing it with reference spectra. In this submitted work, a concept is presented with an example of a “nipple rubber” eluate. It shows that this rubber article liberates a vulcanization accelerator, 2-mercaptobenzothiazole.