Deponiesickerwässer: Bestimmung zytotoxischer Wirkungen mit dem Pollenschlauchwachstumstest |
| |
Authors: | Klaus Jung Udo Kristen Johannes Flachowsky Helmut Segner Gerrit Schüürmann |
| |
Institution: | 1. UFZ-Umweltforschungszentrum Leipzig-Halle, Sektion Chemische ?kotoxikologie, Postfach 2, D-04301, Leipzig 2. Institut für Allgemeine Botanik, Universit?t Hamburg, Ohnhorststr. 18, D-22609, Hamburg 3. Sektion Analytik, UFZ-Umweltforschungszentrum Leipzig-Halle, Postfach 2, D-04301, Leipzig
|
| |
Abstract: | For risk assessment of environmental samples, rapid and sensitive screening assays for detection of toxic potencies are required. Using leachates from waste deposites in the industrial region Leipzig-Halle (Germany) as an example, we present a simple test procedure suitable for the detection of cytotoxic effects of samples. This method, the pollen tube growth test (PTG), works as follows: Pollen of tobacco (Nicotiana sylvestris Spegazz, & Comes) are suspended in mixtures of growth medium and buffered (pH 6,3 to 7,0) dumping ground leachates. After an 18h-incubation period, growth of pollen tubes is quantified by staining with Alcian blue and subsequent measurment of optical density in a photometer. Sample induced inhibition of pollen tube growth is calculated in relation to controls maintained in pure growth medium. The PTG test shows high sensitivity towards leachates of different chemical composition, and it shows good agreement with assay results from luminescent bacteria tests. We conclude that the PTG test is well suited to assess the hazardous potential of aqueous environmental samples. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|