发酵培养法筛选堆肥中产表面活性物质的菌种

微生物在城市生活垃圾堆肥中起着重要的作用 ,而某些微生物在一定条件下产生的生物表面活性剂有望改善堆肥的微环境 ,提高堆肥的效率。从不同温度的堆肥过程中 (45℃和 55℃ )筛选产生物表面活性剂的细菌 ,生物表面活性剂的产生通过测定其发酵液的张力值来判断。实验结果表明 ,堆肥过程中的确存在产生物表面活性剂的细菌 ,枯草芽孢杆菌是其主要菌之一。实验通过一系列培养条件的优化 ,使所筛选到的细菌产生活性较强、浓度较高的生物表面活性剂

Isolating Biosurfactant-Producing Bacteria from Composting

Microorganisms act as an important role in composting of municipal solid waste, while biosurfactant produced by the certain microorganisms may potentially improve this micro-environment and enhance solid waste composting. The study was performed to isolate biosurfactant-producing bacteria from composting at different temperature, 45℃ and 55℃, biosurfactant's producing was determined by measuring the surface tension of fermentation. Experimental results indicated that there really were biosurfactant-producing bacteria and Bacillus subtilis were the dominant biosurfactant-producing bacteria during composting. In addition, through optimizing the culture medium, the isolated bacteria produced strong activity and high concentration biosurfactant.