降解对苯二甲酸厌氧微生物群快速驯化与富集技术

来自甲烷发酵的厌氧污泥在反硝化条件下 ,可以对苯二甲酸 ( Terephthalic Acid,简称 TA)为唯一碳源快速驯化富集 TA厌氧降解菌 ,然后转换到甲烷发酵状态 ,到第 6周时 ,获得的 TA降解速度为 2 2 .6mg TA/g VSS· day,是对照实验的 2 .1倍。经过约 90天的驯化 ,原存在于种污泥中的发酵性细菌几乎全部消亡 ,新的培养物中取而代之的是 TA还原和开环菌 ,TA降解菌的驯化过程和富集过程是偶联的。MPN计数和滚管计数进一步证实了上述结果。

Quick enrichment & acclimation of an anaerobic microbial consortium used for biodegrading terephthalic acid(TA)

The seed sludge originated from a methane fermentation reactor can be enriched and acclimated quickly with TA as sole carbon source for TA anaerobic degradation under nitrate respiration mode first,and then under methane fermentation mode.Through 6-week process,a 22.6mgTA/gVSS*day TA degradation rate was obtained,which was 2.1 times of the rate got from normal acclimation process.After a total of 90 days for the enrichment and acclimation,the fermentative bacteria which originally existed in the seed sludge nearly disappeared,and instead of them,the TA reductive and cleaving bacteria group was formed in the new consortium,which was confirmed by the MPN counts and roll tube counts.