| 流加发酵及添加L-半胱氨酸对产朊假丝酵母高密度培养合成谷胱甘肽的影响 |
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| 引用本文： | 张文燕,堵国成,陈坚. 流加发酵及添加L-半胱氨酸对产朊假丝酵母高密度培养合成谷胱甘肽的影响[J]. 应用与环境生物学报, 2007, 13(2): 261-264 |
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| 作者姓名： | 张文燕 堵国成 陈坚 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏无锡,214036 |
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| 基金项目： | 教育部高等学校优秀青年教师教学科研奖励计划;江苏省高校高新技术产业发展项目 |
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| 摘 要： | 通过7L罐考察了不同葡萄糖流加速率以及添加L-半胱氨酸对产朊假丝酵母(Candida utilis)WSH02-08高密度发酵生产谷胱甘肽(GSH)的影响.结果表明,在恒定速度(5.5g L-1h-1)流加葡萄糖30h的基础上,发酵45h后细胞干重最高达到73g L-1,此时向发酵罐中一次性添加L-半胱氨酸40mmol L-1,最终GSH产量和胞内GSH含量分别达到了1458mg L-1和2.26%.图4表1参11
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| 关 键 词： | 谷胱甘肽 高密度培养 产朊假丝酵母 流加发酵 L-半胱氨酸 |
| 收稿时间： | 2005-11-14 |
| 修稿时间： | 2006-03-06 |
Effects of Fed-batch Culture and L-cysteine Addition on Glutathione Production from High-density Fermentation by Candida utilis |
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| ZHANG Wenyan,DU Guocheng,CHEN Jian. Effects of Fed-batch Culture and L-cysteine Addition on Glutathione Production from High-density Fermentation by Candida utilis[J]. Chinese Journal of Applied and Environmental Biology, 2007, 13(2): 261-264 |
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| Authors: | ZHANG Wenyan DU Guocheng CHEN Jian |
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| Affiliation: | Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036, Jiangshu, China |
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| Abstract: | In order to achieve high-density culture of cells and high yield of glutathione,different glucose feeding rates in fed-batch cultures of Candida utilis WSH 02-08 were investigated in a 7 L stirred fomenter.When glucose was fed at a constant rate of 5.5 g L-1 h-1 for 30 h,the maximum dry cell weight reached 73 g L-1 after 45 h and then 40 mmol L-1 of L-cysteine was added,GSH concentration and intracellular GSH content reached 1458 mg L-1 and 2.26 %,respectively.Fig 4,Tab 1,Ref 11 |
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| Keywords: | glutathione high density cell culture Candida utilis fed-batch culture L-cysteine |
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