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Using the Flow Cytometry to Quantify the Giardia Cysts and Cryptosporidium Oocysts in Water Samples |
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| Authors: | Bing-Mu Hsu Nan-Min Wu Hung-Der Jang Feng-Cheng Shih Min-Tao Wan Chien-Min Kung |
| Institution: | (1) Department of Bioenvironmental Engineering, Chung Yuan Christian University, Chung-Li, Taiwan, ROC;(2) Department of Environmental Engineering and Health, Yuanpei University of Science and Technology, Hsinchu, Taiwan, ROC;(3) Department of Food Science, Yuanpei University of Science and Technology, Hsinchu, Taiwan, ROC;(4) Department of Medical Technology, Yuanpei University of Science and Technology, Hsinchu, Taiwan, ROC |
| Abstract: | The flow cytometry (FC) has been used to detect Giardia cysts and Cryptosporidium oocysts quantitatively and instantaneously in this study. The experimental results showed that FC is potential to become a more precise method for the detection of Giardia and Cryptosporidium in water. This study also evaluated the staining efficiencies for three commercial antibodies. After staining Cryptosporidium oocysts with direct immunofluorescent antibodies in water samples, two populations were detected in the scatter-plots (FL1 versus SSC) of the FC. The Cryptosporidium oocysts and Giardia cysts are significantly separated from other particles while stained with direct immunofluorescent antibodies produced by Meridian Diagnostics and WaterborneTM Inc. |
| Keywords: | Cryptosporidium flow cytometry Giardia protozoan parasites |
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