Physical factors negatively affecting evaluation of long-term biodegradation experiments of polychlorinated biphenyls |
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| Authors: | P Zachǎr ? Novotny? Z Vozňáková M Matucha E Tesa?ová D Sýkora A Kubátová M Popl V Šašek |
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| Institution: | aInstitute of Chemical Technology, Department of Analytical Chemistry, Technická 5, Prague, 166 28, Czech Republic;bInstitute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic;cInstitute of Experimental Botany, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic |
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| Abstract: | Behaviour of PCB added in the form of a technical mixture (Delor 106) and 14C]PCB 77 into different aqueous model cultivation media in long-term experiments was studied. The work was focused on monitoring PCB recovery from liquid media in the absence of micro-organisms. GC-ECD and radioanalysis were utilised for determination of PCB in the examined systems. Strong relations between duration of experiments, composition of media, degree of chlorination of biphenyls, and recovery of PCB were found. The lowest yields of PCB were observed for the extracts from model solutions based on N-limited cultivation media without veratryl alcohol and Tween 80. The addition of these components, mainly of Tween 80, caused a significant increase of PCB recoveries. |
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