An interaction of extra- and intracellular osmoregulatory mechanisms in the bivalve mollusc Rangia cuneata

We have studied the interaction of extra- and intracellular osmoregulatory mechanisms which the estuarine bivalve Rangia cuneata utilize to tolerate salinities ranging from freshwater to 25 ppt. The normally occurring intracellular free amino acid (FAA) accumulation in hyperosmotically stressed R. cuneata isolated ventricles was significantly reduced in the presence of divalent cation concentrations lower than that normally found in the blood. The effect of the divalent cations was not on the intracellular biosynthetic pathways responsible for FAA synthesis but rather on the myocardial membrane permeability to FAA. These data indicate that the effectiveness of the intracellular FAA mediated volume regulatory response in R. cuneata is dependent on both intracellular FAA accumulation and on the extracellular regulation of blood divalent cation concentration. This dependency indicates one interaction of extra- and intracellular osmoregulation in R. cuneata.