| 镉诱导仔猪睾丸支持细胞氧化酶活性降低及DNA损伤的研究 |
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| 引用本文： | 张明,邬静,邓思君,袁莉芸,郭诚志,朱丽,袁慧.镉诱导仔猪睾丸支持细胞氧化酶活性降低及DNA损伤的研究[J].生态环境,2010,19(3):576-579. |
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| 作者姓名： | 张明 邬静 邓思君 袁莉芸 郭诚志 朱丽 袁慧 |
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| 作者单位： | 1. 湖南农业大学动物医学院,湖南,长沙,410128;江西生物科技职业学院,江西,南昌,330200
2. 湖南农业大学动物医学院,湖南,长沙,410128 |
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| 基金项目： | 湖南农业大学青年基金项目，湖南农业大学动物医学院青年基金项目 |
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| 摘 要： | 以仔猪睾丸支持细胞为实验模型，采用二步酶消化法分离支持细胞进行培养。探讨了0、10、20、40、80gmol·L^-1的氯化镉对支持细胞的毒性作用。结果表明：10gmol·L^-1以上的氯化镉有抑制支持细胞生长的作用，并能使支持细胞氧化酶活性下降，造成支持细胞DNA损伤。
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| 关 键 词： | 氯化镉 支持细胞 氧化酶 DNA损伤 |
DNA damage and decrease of cellular oxidase activity in Piglet Sertoli cells exposed to cadmium |
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| ZHANG Ming,WU Jing,DENG Sijun,YUAN Liyun,GUO Chengzhi,ZHU Li,HUI Yuan.DNA damage and decrease of cellular oxidase activity in Piglet Sertoli cells exposed to cadmium[J].Ecology and Environmnet,2010,19(3):576-579. |
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| Authors: | ZHANG Ming WU Jing DENG Sijun YUAN Liyun GUO Chengzhi ZHU Li HUI Yuan |
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| Institution: | 1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China; 2. Jiangxi Biotech Vocational College, Nanchang 330200, China) |
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| Abstract: | The study was designated to explore the toxic effects of cadmium on piglet Sertoli cells. Sertoli cells were isolated from piglet testes uisng a two-step enzyme digestion and followed by differential plating. Piglet Sertoli cells were cultured and classified into five groups, i.e, group A, control without cadmium chloride; group B with 10 μM cadmium chloride; group C with 20 μM cad-mium chloride; group D with 40 μM cadmium chloride; and group E with 80 μM cadmium chloride. We found that Sertoli cells' growth was inhibited by arsanilic acid at 10 μM compared with the control group A. The oxidase activity of Sertoli cells was de-creased by arsanilic acid at 10 μM. Moreover, DNA damage of Sertoli cells was observed by arsanilic acid at 10 μM. |
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| Keywords: | cadmium chloride sertoli cells oxidase DNA damage |
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