Horseradish peroxidase microcapsules based on layer-by-layer polyelectrolyte deposition |
| |
Authors: | Zixia ZHAO Qiang CHEN Jun-ichi ANZAI |
| |
Institution: | 1. Department of Radiology, Thomas Jefferson University, 1020 Locust Street, JAH 474, Philadelphia PA 19107, USA;2. Department of Biochemistry & Molecular Biology, Thomas Jefferson University, Philadelphia PA 19107, USA;3. Department of Medical Oncology, Thomas Jefferson University, Philadelphia PA 19107, USA;4. Department of Kimmel Cancer Center, Thomas Jefferson University, Philadelphia PA 19107, USA;5. Molecular Targeting Technologies, West Chester PA 19380, USA |
| |
Abstract: | Polyelectrolyte multilayer microcapsules have been prepared for encapsulating horseradish peroxidase (HRP). The CaCO3 microparticles containing HRP molecules were prepared and subsequently enwrapped with layer-by-layer (LbL) deposited multilayer films. The LbL films were constructed via an alternate electrostatic adsorption of poly(allylamine) and poly(styrene sulfonate). The CaCO3 templates were then dissolved in an ethylenediaminetetraacetic acid solution, leaving HRP in the microcapsules. Optical microscope and fluorescence microscope observations indicated that the microcapsules are homogeneous spheres with an average diameter about 7 μm. The encapsulated HRP showed catalytic activity in the oxidation of pyrogallol, suggesting the microcapsules provide a suitable environment for enzymatic reaction. A leakage of HRP from the microcapsules was negligibly small. Therefore, the HRP-containing microcapsules is promising for detection and treatment of phenolic compounds. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|