Production of iturin A homologues under different culture conditions |
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| Authors: | Noriyasu IWASE Mohammad Shahedur RAHMAN Takashi ANO |
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| Institution: | 1. CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;2. State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China;3. Shanghai-MOST Key Laboratory for Health and Disease Genomics, Chinese National Human Genome Center, Shanghai, China;4. Department of Microbiology and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region;1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;2. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, China;3. Department of Chemistry, State University of New York-College of Environmental Science and Forestry, Syracuse, NY 13210, USA;4. Lifecome Biochemistry Stock CO. Ltd., Pucheng 353400, China;1. Laboratoire des Procédés Biologiques, Génie Enzymatique et Microbien-ProBioGEM, UPRES-EA 1026, Polytech’Lille/IUT A, Université Lille Nord de France-Sciences et Technologies, F-59655 Villeneuve d’Ascq Cedex, France;2. Axe GePEB, Institut Pascal, UMR 6602, Université Blaise Pascal, CNRS, Clermont Université, BP 10448, F-63000 Clermont-Ferrand, France;3. Unité de Biotechnologies et Bioprocédés, Université Libre de Bruxelles, Av. F.-D. Roosevelt 50, C.P. 165/61, B-1050 Bruxelles, Belgium;4. Unité de Chimie Biologique Industrielle, Gembloux Agro-BioTech, Université de Liège, B-5030 Gembloux, Belgium;1. Key Laboratory of Industrial Biotechnology of Ministry of Education, State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China;2. Suqian Industrial Technology Research Institute of Jiangnan University, Suqian 223800, China |
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| Abstract: | Iturin A is a cyclic lipopeptide antibiotic and eight different kinds of iturin A have been reported based on its alkyl side chains. As iturin A is a promising biocontrol agent, total production of iturin A was tried to enhance and comparative production of its homologues was investigated by using different nitrogen and carbon sources. When Polypepton S and defatted soybean meal were used, total production as well as the ratio of the iturin A homologues were similar. However, production of iturin A was relatively lower and also the ratio of the iturin A homologues was different when Polypepton was used, where A2 was decreased and A4 was increased. Production ratio of the iturin A homologues was similar for the carbon sources like maltose, mannitol, sucrose and starch but relative production of iturin A2 was much enhanced compared to A3 when lactose or galactose was used. Interestingly production ratio of A4 was increased and A2 and A3 were decreased when no additional carbon source was used, and similar tendency was observed in the homologue ratio with glucose and fructose. Production of iturin A homologue A6 was significantly increased whereas A2 and A3 were decreased when defatted rapeseed cake was used. Utilization of different amino acids did not show significant differences in their production of the iturin A homologues. Oxygen supply found to be the factor affecting the production of iturin A homologues when it was investigated in a varied culture volume size and shaking speed. A2 found to be increased with increased oxygen supply where the production of A3 was affected inversely. |
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