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耐盐好氧反硝化菌筛选及其反硝化特性的研究
引用本文:李静,王文文,梁磊,冯文清. 耐盐好氧反硝化菌筛选及其反硝化特性的研究[J]. 环境科学与技术, 2011, 34(6): 48-52. DOI: 10.3969/j.issn.1003-6504.2011.06.011
作者姓名:李静  王文文  梁磊  冯文清
作者单位:1. 广东轻工职业技术学院,广东广州,510300
2. 广州甘蔗糖业研究所,广东广州
3. 广州奥桑味精食品有限公司,广东广州,510280
摘    要:为了筛选耐盐好氧反硝化菌,对活性污泥进行耐盐与好氧反硝化驯化后,分离得到具有很强反硝化能力的菌株GQ-42,经菌落形态特征观察、生理生化测定及16S rDNA测序及同源性比较等过程,鉴定为蜡状芽孢杆菌.通过对菌株GQ-42进行反硝化特性的研究,确定适宜的反硝化条件为:碳氮比≥6,温度26~38℃,pH6.0~9.0,溶...

关 键 词:好氧反硝化  筛选  耐盐  蜡状芽孢杆菌

Screening of Aerobic Denitrifying Bacteria with Salt To1erance and Characteristics of Aerobic Denitrification
LI Jing,WANG Wen-wen,LIANG Lei,FENG Wen-qing. Screening of Aerobic Denitrifying Bacteria with Salt To1erance and Characteristics of Aerobic Denitrification[J]. Environmental Science and Technology, 2011, 34(6): 48-52. DOI: 10.3969/j.issn.1003-6504.2011.06.011
Authors:LI Jing  WANG Wen-wen  LIANG Lei  FENG Wen-qing
Affiliation:LI Jing1,WANG Wen-wen1,LIANG Lei2,FENG Wen-qing3(1.Guangdong Light Industry Technical College,Guangzhou 510300,China,2.Guangzhou Sugarcane and Canesugar Research Institute,Guangzhou 510316,3.Guangzhou Aosang Monosodium Glutamate Food Limited Company,Guangzhou 510280,China)
Abstract:In order to screen aerobic denitrifying bacteria with salt tolerance,strain GQ-42 was isolated from activated sludge because of the best denitrifying capability.Based on morphology and physiology of colony as well as 16S rDNA sequence analysis,the strain was characterized as Bacillus cereus.The favorable condition of aerobic denitrification for strain GQ-42 was determined as C/N more than 6,temperature 26~38 ℃,pH 6.0~8.0,dissolved oxygen concentration 5.4~8.5 mg/L and salinity less than 150 g/L.On the condition,all of removal rate of NO3——N were more than 90%,with the best removal rate of NO3——N as 93.77%.
Keywords:aerobic denitrification  screening  salt-tolerant  Bacillus cereus  
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