| 痕量磺胺二甲基嘧啶ELISA与HPLC检测方法比较 |
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| 引用本文： | 田世烜,张萌,陈亮,黄满红.痕量磺胺二甲基嘧啶ELISA与HPLC检测方法比较[J].环境科学与技术,2011,34(6):115-118. |
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| 作者姓名： | 田世烜 张萌 陈亮 黄满红 |
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| 作者单位： | 东华大学环境科学与工程学院,上海,201620 |
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| 基金项目： | 高等学校博士点专项科研基金(20090075120007); 上海科委项目(09230500200); 中央高校基本科研业务费专项资金项目(10D11308) |
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| 摘 要： | 比较了城市污水中磺胺二甲基嘧啶(SM2)的两种检测方法,即酶联免疫(ELISA)法和SPE-HPLC法.HPLC法以Agilent ZORBAX Exlipse XDB-C18色谱柱为分析柱,流动相乙腈与超纯水的比例为2:8(V:V),270nm处检测SM2含量.结果表明,ELISA法的平均回收率为90.4%,相对标准...
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| 关 键 词： | ELISA HPLC 磺胺二甲基嘧啶(SM2) 城市污水 检测 |
Comparison of ELISA and HPLC for Trace Sulfamethazine Determination in Municipal Sewage |
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| TIAN Shi-xuan,ZHANG Meng,CHEN Liang,HUANG Man-hong.Comparison of ELISA and HPLC for Trace Sulfamethazine Determination in Municipal Sewage[J].Environmental Science and Technology,2011,34(6):115-118. |
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| Authors: | TIAN Shi-xuan ZHANG Meng CHEN Liang HUANG Man-hong |
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| Institution: | TIAN Shi-xuan,ZHANG Meng,CHEN Liang*,HUANG Man-hong(School of Environmental Science and Engineering,Donghua University,Shanghai 201620,China) |
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| Abstract: | Enzyme-linked immunosorbent assay(ELISA)was compared with high performance liquid chromatography(HPLC)for trace determination of sulfamethazine in municipal sewage.For HPLC,Agilent ZORBAX Exlipse XDB-C18 column was applied for separation of the investigated sulfamethazine.Acetonitrile and ultrapure water were used as the mobile phase,and the wavelength of determination was 270 nm.Results showed that average recovery of ELISA was 90.47%,RSD was 2.6% and limit of detection was 4 ng/mL.HPLC average recovery of... |
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| Keywords: | ELISA HPLC sulfamethazine(SM2) municipal sewage detection |
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