毒死蜱对雄性小鼠生殖毒性的影响

为研究毒死蜱对雄性小鼠生殖毒性的影响,利用不同浓度毒死蜱染毒小鼠.以昆明小鼠为受试动物,毒死蜱按3、6和12mg·kg-13个剂量水平,灌胃染毒小鼠7d.以睾丸组织匀浆测定活性氧(reactive oxygen species,ROS)和还原型谷胱甘肽(glutathione,GSH)的含量；以睾丸细胞测定DNA-蛋白...

Effect of Reproductive Toxicity Induced by Chlorpyrifos on Male Mice

To research the effect of reproductive toxicity of chlorpyrifos on male mice which were gavage with different concerntrations of chlorpyrifos. Male mice were orally administrated with chlorpyrifos daily for 7 days at the concentrations of 3, 6 and 12 mg·kg-1, respectively. Afterwards, the contents of ROS (reactive oxygen species) and GSH (glutathione) in the testis tissue homogenates, and DPC (DNA-protein crosslink) coefficients in the testis cells were measured. Testis tissue sections were made then stained for pathological observations under microscope. Results showed that the contents of ROS and DPC coefficients gradually increased in a dose-dependent manner, whereas GSH content decreased. In the exposure group, with the dose of 6 mg kg-1, GSH content was significantly higher than that of the control group (p< 0.05);ROS contents had extremely significant differences (p<0.01). Stained sections presented the increased cavity surface in the testicular seminiferous tubules. Sperm and sperm generation associated cells were obviously reduced. In the higher dose groups (12 mg·kg-1), the differences of DPC, ROS, and GSH were all significant (p< 0.01). Within the testicular seminiferous tubules, elongations of sperm cells were blocked; mature sperms were hardly found; spermatogenic cells apoptosis and stromal cell proliferation were observed. It can be concluded that chlorpyrifos at higher doses(≥6 mg·kg-1) can increase the oxidative stress and pathological damagein male mouse testis, which induced the reproductive toxicity .