Inhibition of animal acetyl-coenzyme a carboxylase by 2-(p-chlorophenoxy)-2-methylpropionic acid and 2-ethylhexanoic acid |
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| Affiliation: | 1. Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China;2. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China;3. Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China;4. University of Chinese Academy of Sciences, Beijing, 100049, PR China;1. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China;2. Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300350, PR China;3. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300350, PR China;1. College of Food Science and Technology, Nanchang University, 999 Xuefu Road, Nancang 330031, China;2. School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing 210023, China;3. Jiangxi New Reyphon Biochemical Co., Ltd., Salt and Chemical Industry, Xingan, China;4. Ministry of Education Key Laboratory of NSLSCS, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing 210023, China;1. National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, 214122, China;2. Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China;3. School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China;4. Jiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China;5. Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang, 550025, China;6. Dalian Research Institute of Petroleum and Petrochemicals, SINOPEC, Dalian, 116000, China;7. School of Chemical Engineering, Dalian University of Technology, Dalian, 116000, China;1. Biochemistry and Molecular Biology, University of California, Riverside, Riverside, CA, USA;2. Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA, USA;3. Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA;4. Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, USA;5. Center for Industrial Biotechnology, University of California, Riverside, Riverside, CA, USA |
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| Abstract: | Peroxisome proliferators are generally known as activators of fatty acid β-oxidation which is one of degradation pathways. However, since it is unknown whether peroxisome proliferators have an effect on biosynthesis of fatty acid or not, we commenced to study if acetyl-CoA carboxylase, the rate-limiting enzyme of fatty acid biosynthesis, is inhibited by 2-(p-chlorophenoxy)-2-methylpropionic acid or 2-ethylhexanoic acid which is a typical peroxisome proliferator. Acetyl-CoA carboxylase(s) from rat, mouse, hamster, rabbit, dog and monkey were completely inhibited by 2-(p-chlorophenoxy)-2-methylpropionic acid or 2-ethylhexanoic acid at the concentration of 10−3M. For both compounds, there was not a large species difference in the inhibition. However, there was only several times difference between I50 values for the two compounds. The findings imply that inhibition of acetyl-CoA carboxylase by the chemicals leads to reduction of fatty acid biosynthesis when the chemicals are administered to the animals at high dose levels. |
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