| 一株烟草内生拮抗放线菌发酵条件优化 |
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| 引用本文： | 孙会强,周赛男,谭周进,伍兵,谢丙炎,申可佳.一株烟草内生拮抗放线菌发酵条件优化[J].应用与环境生物学报,2011(6):914-917. |
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| 作者姓名： | 孙会强 周赛男 谭周进 伍兵 谢丙炎 申可佳 |
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| 作者单位： | 湖南农业大学生物安全科学技术学院;湖南中医药大学基础医学院;中国农业科学院蔬菜花卉研究所; |
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| 基金项目： | 科技部支撑计划课题(No.2006BAD08A08); 长沙市科技局课题(No.K1104070-31)资助~~ |
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| 摘 要： | 为提高烟草内生放线菌Y12发酵时产生抑菌物质的产量,通过摇瓶正交优化试验,确定了Y12的三角瓶发酵条件.发酵培养基组成为:黄豆粉1.5%,蛋白胨1.5%,葡萄糖1.5%,可溶性淀粉1.5%,MgSO4.7H2O 0.05%,(NH4)SO40.25%,NaCl 0.4%,KH2PO4 0.1%,CaCO3 0.5%;培养基初始pH 7.5,培养温度30℃,发酵时间72 h.经过优化发酵液的抑菌圈直径增加了44.9%.
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| 关 键 词： | 内生放线菌 抑菌作用 正交试验 发酵优化 |
Optimization of Fermentation Conditions of an Endophytic Actinomycetes from Tobacco Plant |
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| SUN Huiqiang,ZHOU Sainan,TAN Zhoujin,WU Bing,XIE Bingyan & SHEN Kejia.Optimization of Fermentation Conditions of an Endophytic Actinomycetes from Tobacco Plant[J].Chinese Journal of Applied and Environmental Biology,2011(6):914-917. |
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| Authors: | SUN Huiqiang ZHOU Sainan TAN Zhoujin WU Bing XIE Bingyan & SHEN Kejia |
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| Institution: | SUN Huiqiang1,2,ZHOU Sainan2,TAN Zhoujin2,WU Bing1,XIE Bingyan3 & SHEN Kejia2(1School of Biosafety Science and Technology,Hunan Agricultural University,Changsha 410128,China)(2School of Preclinical Medicine,Hunan University of Chinese Medcine,Changsha 410208,China)(3Institute of Vegetable and Flower,China Academy of Agricultural Sciences,Beijing 100081,China) |
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| Abstract: | In order to increase the yield of the antibacterial substance produced by an endophytic actinomycetes,Y12,from tobacco plant in the process of fermentation,a series of orthogonal experiments on Y12 were carried out in the shaking flasks to obtain optimal components of the fermentation media as well as the fermentation conditions.It was concluded that the optimal proportion of the fermentation medium was: 1.5% soybean flour,1.5% peptone,1.5% glucose,1.5% soluble starch,0.05% MgSO4.7H2O,0.25%(NH4)SO4,0.4% NaC... |
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| Keywords: | endophytic actinomycetes inhibition function orthogonal experiment fermentation condition optimization |
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