| 皮氏罗尔斯通氏菌株DX-T3-01的耐镉性能及镉富集机理 |
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| 引用本文： | 付瑾,谢学辉,钱林,柳建设.皮氏罗尔斯通氏菌株DX-T3-01的耐镉性能及镉富集机理[J].应用与环境生物学报,2011(5):717-721. |
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| 作者姓名： | 付瑾 谢学辉 钱林 柳建设 |
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| 作者单位： | 东华大学环境科学与工程学院; |
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| 基金项目： | 全国优秀博士学位论文作者专项资金项目(No.200549); 国家自然科学基金项目(No.50874032); 上海市重点学科建设项目(No.B604)资助~~ |
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| 摘 要： | 从江西德兴铜矿土壤中分离筛选到一株具有较强耐镉(Cd)能力的细菌DX-T3-01,经生理生态及16S rRNA分析进行鉴定,研究其对不同浓度Cd2+的耐受特性及对重金属Zn2+、Cu2+、Ni2+的耐受性,进一步利用扫描电镜-能谱仪(SEM-EDX)和红外光谱(FTIR)探讨菌株对Cd2+累积去除的机理.经鉴定,耐镉细菌DX-T3-01为皮氏罗尔斯通氏菌(Ralstonia pickettii);Cd2+最高耐受浓度(MTC)为16 mmol L-1,最低抑制浓度(MIC)为4 mmol L-1,并可耐受一定浓度的Zn2+、Cu2+;菌体积累Cd2+后细胞表面有沉淀物附着并有镉元素检出,对Cd2+的累积主要依靠细胞壁上—PO43-、胺基中的—C—N—、—M—O(O—M—O)、C=O和酰胺基(—CO—NH—)基团.图5参16
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| 关 键 词： | 皮氏罗尔斯通氏菌 菌株筛选 16SrRNA 耐镉特性 红外光谱 |
Cadmium Tolerance and Bio-accumulation Mechanisms of Ralstonia pickettii strain DX-T3-01 |
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| FU Jin,XIE Xuehui,QIAN Lin & LIU Jianshe.Cadmium Tolerance and Bio-accumulation Mechanisms of Ralstonia pickettii strain DX-T3-01[J].Chinese Journal of Applied and Environmental Biology,2011(5):717-721. |
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| Authors: | FU Jin XIE Xuehui QIAN Lin & LIU Jianshe |
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| Institution: | FU Jin,XIE Xuehui,QIAN Lin & LIU Jianshe(College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China) |
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| Abstract: | A cadmium-resisting bacterium,strain DX-T3-01,was isolated from the tailing dam of Dexing Copper Mine in Jiangx,China,and its tolerance to cadmium was investigated in liquid medium with different Cd2+ concentrations.Besides,the resistance of the strain to other heavy metals such as Zn2+,Cu2+ and Ni2+ were also studied.The mechanism of cadmium accumulation by stain DX-T3-01 was evaluated by scanning electron microscopy-energy dispersive X-ray spectroscopy(SEM-EDX) and infrared spectroscopy(FTIR).The strain w... |
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| Keywords: | Ralstonia pickettii strain isolation 16S rRNA cadmium tolerance FTIR |
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