| 烷烃降解菌的筛选、鉴定及优势菌株的降解特性 |
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| 引用本文： | 郭娜,李志敏,叶勤.烷烃降解菌的筛选、鉴定及优势菌株的降解特性[J].应用与环境生物学报,2011(4):572-576. |
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| 作者姓名： | 郭娜 李志敏 叶勤 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室; |
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| 基金项目： | 发酵工程上海市重点学科项目(No.B505)资助~~ |
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| 摘 要： | 以正庚烷为唯一碳源,从长期受到石油污染的土壤中筛选获得可利用正庚烷的微生物14株.通过形态观察和16S rDNA序列比对,鉴定G2、G9、G14为红球菌属,G3、G27为人苍白杆菌属,G4、G7为芽孢杆菌属,G5、G10、G15、G25为节杆菌属,G16为缺陷短波单胞菌,G17、G22为嗜麦芽寡养单胞菌属.通过考察其降解烷烃的能力,确定Rhodococcus sp.G2为烷烃降解优势菌株.该菌株可代谢庚烷获得最大菌体浓度D600 nm=7.51.同时该菌对不同碳链长度的烷烃,如十二烷、十六烷、煤油和二甲苯均具有较强的降解能力,以十二烷为碳源的最大比生长速率为0.37 h-1,最高菌体浓度为D600 nm=12.00,在正十六烷中生长,最大比生长速率为0.23 h-1,在煤油中生长,最大比生长速率为0.14 h-1,在以二甲苯为唯一碳源时,D600 nm也可达到1.00左右.研究表明该菌株对于石油污染土壤的生物修复有很大的应用前景.图6表2参9
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| 关 键 词： | 烷烃降解 红球菌 菌株筛选 16SrDNA 石油污染土壤 生物修复 |
Screen and Identification of Alkane Degrading Bacteria and Characteristics of a Predominant Strain |
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| GUO Na,LI Zhimin & YE Qin.Screen and Identification of Alkane Degrading Bacteria and Characteristics of a Predominant Strain[J].Chinese Journal of Applied and Environmental Biology,2011(4):572-576. |
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| Authors: | GUO Na LI Zhimin & YE Qin |
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| Institution: | GUO Na,LI Zhimin & YE Qin(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China) |
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| Abstract: | Using n-heptane as sole carbon source,14 strains were enriched and isolated from oil-polluted soil,and their potential in bioremediation was investigated.The results of 16S rDNA sequence analysis and morphological observation showed that G2,G9 and G14 belonged to the genus Rhodococcus;G3 and G27 belonged to Ochrobactrum;G4 and G7 belonged to Bacillus;G5,G10,G15,and G25 belonged to Arthrobacter;G16 belonged to Brevundimonas diminuta;and G17 and G22 belonged to Stenotrophomonas.Among them,Rhodococcus sp.G2 wa... |
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| Keywords: | alkane-degrading bacterium Rhodococcus strain screen 16S rDNA oil-polluted soil bioremediation |
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