| 华重楼内生菌Iun35的分离及其抗菌蛋白的性质 |
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| 引用本文： | 雍彬,张超,马沁沁,李佳,王一丁. 华重楼内生菌Iun35的分离及其抗菌蛋白的性质[J]. 应用与环境生物学报, 2011, 0(4): 537-540 |
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| 作者姓名： | 雍彬 张超 马沁沁 李佳 王一丁 |
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| 作者单位： | 四川师范大学生命科学学院; |
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| 基金项目： | 国家自然科学基金项目(No.30471378)资助~~ |
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| 摘 要： | 从华重楼(Paris polyphylla Smith var.chinensis)块茎中分离纯化得到一株具有较强抑菌活性的内生细菌Iun35,经形态特征、生理生化特性及16S rDNA鉴定为枯草芽孢杆菌(Bacillus subtilis).将分离得到的Iun35菌株发酵培养,培养液上清经硫酸铵分级沉淀、Sephadex G-75凝胶柱层析和DEAE-32纤维素柱层析分离纯化出一种抗菌蛋白UD35.用该蛋白对多种植物病原菌进行拮抗测定,结果显示,UD35对玉米纹枯病菌(Rhizoctonia solani Kuha)、小麦赤霉病菌[Gibberelle zeae(Schw.)]等多种菌有强烈抑制作用.测定其相对分子质量约为55 000,氨基酸分析结果表明其由17种氨基酸组成.图3表4参16
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| 关 键 词： | 华重楼 内生细菌 抗菌蛋白 分离纯化 抑菌活性 |
Isolation of Endophytic Bacterium Iun35 from Paris polyphylla var. chinensis and Properties of Its Antibiotic Protein |
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| YONG Bin,ZHANG Chao,MA Qinqin,LI Jia , WANG Yiding. Isolation of Endophytic Bacterium Iun35 from Paris polyphylla var. chinensis and Properties of Its Antibiotic Protein[J]. Chinese Journal of Applied and Environmental Biology, 2011, 0(4): 537-540 |
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| Authors: | YONG Bin ZHANG Chao MA Qinqin LI Jia & WANG Yiding |
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| Affiliation: | YONG Bin,ZHANG Chao,MA Qinqin,LI Jia & WANG Yiding(College of Sciences,Sichuan Normal University,Chengdu 610068,China) |
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| Abstract: | Endophytic bacteria from Paris polyphylla Smith var.chinensis Hara can produce proteins with strong antibiotic activity.In this research,an active antibiotic endophytic bacterium Iun35 was isolated from the tuber of P.polyphylla var.chinensis.The strain was classified as Bacillus subtilis based on its characteristics of morphology,physiology and biochemistry as well as the 16S rDNA analysis.After(NH4)2SO4 fractional precipitation,SephadexG-75 and DEAE-32 column chromatography,an antibiotic protein UD35 was ... |
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| Keywords: | Paris polyphylla Smith var.chinensis endophyte antibiotic protein isolation and purification antagonistic activity |
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