Preparation of biofilm electrode with Xanthomonas sp. and carbon nanotubes and the application to rapid biochemical oxygen demand analysis in high-salt condition |
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Authors: | Jing Chen Zhiguo Yu Jinfeng Sun Jianbo Jia Genxi Li |
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Institution: | School of Life Science, Shanghai University, Shanghai, PR China. chenjing6910@163.com |
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Abstract: | A Xanthomonas sp. was isolated from the sludge on the drain outlet of a pharmaceutical factory. Then, the bacterium and carbon nanotubes (CNTs) were co-attached to an oxygen electrode for rapid analysis of biochemical oxygen demand (BOD). The response current was linear with BOD values in the range 10 to 300 mg/L for standard BOD solution with a response time of 35 seconds (R = 0.9994) and 20 to 580 mg/L for pharmaceutical wastewater with a response time < or =200 seconds (R = 0.9985), which means that this modified electrode might be used for online BOD analysis of pharmaceutical wastewater. Further studies revealed that the modified electrode can be used for BOD measurement in a high-salt condition. Also, the bacterium/CNTs biofilm can maintain its activity and good performance, even after being sealed and stored at 4 degrees C for 50 days. |
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