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嗜热硫酸盐还原菌的分离及生长影响因素研究
引用本文:万里,郑连爽,陈丽娥,张俊杰,沈中峰,刘金辉,尹丹丹.嗜热硫酸盐还原菌的分离及生长影响因素研究[J].环境科学与技术,2009,32(10).
作者姓名:万里  郑连爽  陈丽娥  张俊杰  沈中峰  刘金辉  尹丹丹
作者单位:武汉理工大学理学院,湖北,武汉,400070
摘    要:从油田污水中分离得到一株嗜热硫酸盐还原菌,并研究了该菌株的形态生理特性,探讨了碳源、还原剂、pH和温度对该菌株的生长和硫酸盐还原能力影响。结果表明:该菌株为革兰氏阳性杆菌,最适合碳源为柠檬酸钠,在加入了硫代硫酸钠作为还原剂的培养基中生长较好,最适合生长pH为中性,最适生长温度为60℃。在最适条件下培养,2d后培养基变黑,菌株的生长在3d后达到最大值,硫化氢的生成在4d后达到最大值。

关 键 词:嗜热硫酸盐  还原菌  菌株分离  生长影响因素

Isolation of Thermophilic Sulfate Reducing Bacteria and Study on Its Growth Affecting Factors
WAN Li,ZHENG Lian-shuang,CHEN Lie,ZHANG Jun-jie,SHEN Zhong-feng,LIU Jin-hui,YIN Dan-dan.Isolation of Thermophilic Sulfate Reducing Bacteria and Study on Its Growth Affecting Factors[J].Environmental Science and Technology,2009,32(10).
Authors:WAN Li  ZHENG Lian-shuang  CHEN Lie  ZHANG Jun-jie  SHEN Zhong-feng  LIU Jin-hui  YIN Dan-dan
Institution:School of Science;Wuhan University of Technology;Wuhan 430070;China
Abstract:A strain of thermophilic sulfate reducing bacteria was isolated from the sewage of an oil field.Morphological and physiological characteristics of the strain were studied,as well as the impact of carbon source,reducing agents,pH and temperature on the strain growth and ability of reducing sulfate.Results indicated that the strain is a Gam-positive bacillus,with sodium citrate as the optimal carbon source,and sodium hyposulfite as the optimal reducing agent,the optimal pH neutral and optimal temperature arou...
Keywords:thermophilic sulfate-reducing bacteria  strain isolation  growth affecting factor  
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