基于Miseq的好氧反硝化菌源水脱氮的种群演变

为了研究好氧反硝化菌源水脱氮过程中水体微生物群落的演变,利用Miseq高通量测序法对投菌和对照两系统水体样本的微生物信息进行统计,并对两组样品进行了优化序列统计,OTU分布统计和分类学分析的基础分析;以及细菌群落结构,PCA,Rank-Abundance,Hcluster,Specaccum和OTU分布的高级分析.结果显示,投加贫营养好氧反硝化菌的源水系统的氮素得到有效去除,脱氮效果明显;层次聚类和主成分分析显示两系统内的群落结构发生变化,投菌系统与对照系统主要表现为变形菌和拟杆菌门;细菌主要门类和水质参数的相关性分析得出,水质指标对两系统群落变化作用明显;与此同时,投菌系统中有关氮循环的细菌有上升的变化过程. Miseq高通量测序研究源水脱氮过程的微生物种群演变可行,为研究原位生物脱氮过程的水体微生物群落演变提供技术支撑.

Bacterial community structures of aerobic denitrification bacteria nitrogen removal process in source water experiment by using Miseq high-throughput sequencing technique.

Miseq high-throughput sequencing technique was used to investage the bacterial community structure of nitrogen removal process in source water experiment systems added aerobic denitrification bacteria. Bioinformatics analysis were carried on the for the samples of bacteria and control systems. Then, the fundamental analysis, Optimized sequence statistics, OTU distribution, and Taxonomic analysis; the Advanced analysis, the microbial community, PCA, Rank-Abundance, Hcluster, Specaccum, and Venn of OTU distribution. As a result, the nitrogen of bacteria system was removed obviously, and Hierarchical cluatering and PCA showed that the microbial community structure of bacteria and control systems has changed, and the Protebacterice and Bacteroidetes were the main phylum in experiment systems. Meanwhile, the N-functional microbial of bacteria system had an increase process, obviously, which was consistent with the changes of aerobic denitrification bacteria. From all the results, the Miseq high-throughput sequencing technique was an effective tool to explore the changes of bacterial community structure of nitrogen removal process, which could supply a reference to study the changes of community structure of remediation the source water in situ.