沉积物与盐度对罗红霉素生物有效性的影响

在河口环境,沉积物与盐度对抗生素的生物有效性有重要影响。使用超高效液相色谱-串联质谱(UPLC-MS/MS)分析单一和复合控制系统(水、水-沉积物、水-斑马鱼、水-沉积物-斑马鱼)中的各相态中的罗红霉素,以期量化各相态中罗红霉素的迁移、分布。随着暴露时间延长,水体中罗红霉素浓度不断减少,沉积物和斑马鱼体内罗红霉素不断蓄积。沉积物的存在会减少罗红霉素在斑马鱼中的生物有效性。在高盐度环境下,沉积物拥有更大的吸附效率,符合盐析效应,导致罗红霉素的生物有效性及斑马鱼富集能力下降。水-沉积物-斑马鱼系统达平衡时,沉积物为罗红霉素的主要富集场所,可积累42.0%的罗红霉素。同时,斑马鱼可吸收和富集0.16%的罗红霉素。上述研究为研究抗生素环境行为和毒理提供了参考。

Effects of Sediment and Salinity on the Bioavailability of Roxithromycin

Sediment and salinity play an important role in the bioavailability of antibiotics in zebrafish in estuarine and coastal environments. The concentrations of roxithromycin in different single or combined systems (water, water-sediment, water-zebrafish and water-sediment-zebrafish) were analyzed by UPLC-MS/MS, to quantify their respective contributions to roxithromycin distribution in the simulated environment. Following exposure, roxithromycin concentrations in water were gradually reduced, while in sediment and zebrafish gradually increased. It was confirmed that the presence of sediment in the water reduced the bioavailability of roxithromycin. Under the condition of high salinity, roxithromycin was more likely to adsorb to sediment as a result of salting out effect, leading to a reduction in roxithromycin bioavailability and body burden of roxithromycin residues in zebrafish. At equilibrium, in the water-sediment-zebrafish system, sediment could adsorb 42.0% of roxithromycin as the main accumulation place. Meanwhile, zebrafish could absorb 0.16% of roxithromycin. The results presented in this study will provide a reference for the study of environmental behavior and toxicology of antibiotics.