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A New Test Method for Determining Biodegradation of Plastic Material Under Controlled Aerobic Conditions in a Soil-Simulation Solid Environment
Authors:Sophie Grima  Véronique Bellon-Maurel  Françoise Silvestre  Pierre Feuilloley
Affiliation:(1) Laboratoire de Chimie Agro-industrielle UMR 1010 INRA/INPT-ENSIACET, Ecole Nationale Supérieure des Ingénieurs en Arts Chimiques et Technologiques, 118 route de Narbonne, 31077 Toulouse cedex, France;(2) Cemagref, 361 rue JF Breton, BP 5095, 34033 Montpellier Cedex 01, France
Abstract:A new test method is described for assessing biodegradation of plastic material under simulated soil conditions. An inert substrate can be activated with soil extract and nutrient and used in place of soil in biodegradation tests. The biodegradation level is evaluated by determining the carbon dioxide (CO2) production released by the test reactors. Effects of substrate nature, solution pH, nutrient composition, soil extract concentration, and activation duration on CO2 production were investigated, and the experimental conditions were optimized. Results obtained with cellulose showed a biodegradation rate of 80% within 28 days. Moreover, with this kind of substrate, reaction products and residues can be easily extracted and analysed.
Keywords:Polymer biodegradation  soil simulation  inert substrate
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