| 丹江口库区表层浮游细菌群落组成与PICRUSt功能预测分析 |
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| 引用本文： | 张菲,田伟,孙峰,陈彦,丁传雨,庞发虎,姚伦广,李玉英,陈兆进.丹江口库区表层浮游细菌群落组成与PICRUSt功能预测分析[J].环境科学,2019,40(3):1252-1260. |
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| 作者姓名： | 张菲 田伟 孙峰 陈彦 丁传雨 庞发虎 姚伦广 李玉英 陈兆进 |
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| 作者单位： | 南阳师范学院农业工程学院,南水北调中线水源区水安全河南省协同创新中心,南阳 473061;环境保护部南京环境科学研究所,南京,210042;南阳师范学院生命科学与技术学院,南阳,473061 |
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| 基金项目： | 国家自然科学基金项目（51879130，41601332，U1704124）；河南省科技攻关计划项目（172102110259，182102110468）；河南省教育厅高等学校重点科研项目（16A210012）；河南省南水北调中线水源区水生态安全创新型科技团队专项（17454） |
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| 摘 要： | 浮游细菌是水生生态系统的重要组成部分,是水体中氮素生物地球化学循环的主要驱动力.本研究于2016年5月采集丹江口库区库心和渠首2个生态位点表层水样,采用16S r DNA Miseq高通量测序技术研究其群落组成,发现其主要由变形菌门、放线菌门、拟杆菌门等12门、139属细菌组成,渠首样品浮游细菌群落多样性高于库心样品. PICRUSt功能预测分析表明,浮游细菌涉及氨基酸运输和代谢、转录、能量产生和转换等24个基因功能家族,表现出功能上的丰富性.其中35个参与氮代谢的KO(表示通路)中库心高于渠首为20个,渠首高于库心的为15个.两样品中检测到涉及固氮作用(nif H)、硝化作用(hao)、反硝化作用(nar G、nir K、nor B、nos Z)、氮同化还原及异化还原作用(nas A、nar B、nap A、nir A、nir B、nrf A)参与氮循环的关键基因相对丰度.综合基因功能家族预测基因拷贝数和氮循环相关基因丰度分析,丹江口库区库浮游细菌氮代谢能力整体趋势为库心高于渠首.本研究从细菌群落组成、功能角度初步分析了丹江口库区不同生态位点氮循环的差异,为丹江口水库水环境保护提供了参考依据.
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| 关 键 词： | 浮游细菌 细菌群落组成 高通量测序 PICRUSt功能预测 氮代谢 |
| 收稿时间： | 2018/6/12 0:00:00 |
| 修稿时间： | 2018/9/2 0:00:00 |
Community Structure and Predictive Functional Analysis of Surface Water Bacterioplankton in the Danjiangkou Reservoir |
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| ZHANG Fei,TIAN Wei,SUN Feng,CHEN Yan,DING Chuan-yu,PANG Fa-hu,YAO Lun-guang,LI Yu-ying and CHEN Zhao-jin.Community Structure and Predictive Functional Analysis of Surface Water Bacterioplankton in the Danjiangkou Reservoir[J].Chinese Journal of Environmental Science,2019,40(3):1252-1260. |
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| Authors: | ZHANG Fei TIAN Wei SUN Feng CHEN Yan DING Chuan-yu PANG Fa-hu YAO Lun-guang LI Yu-ying and CHEN Zhao-jin |
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| Institution: | Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China and Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China |
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| Abstract: | |
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| Keywords: | bacterioplankton bacterial community structure high-throughput sequencing PICRUSt analysis nitrogen metabolism |
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