| 紫色土丘陵区畜禽养殖场土壤中抗生素抗性基因分布特征 |
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| 引用本文： | 程建华,唐翔宇,刘琛.紫色土丘陵区畜禽养殖场土壤中抗生素抗性基因分布特征[J].环境科学,2019,40(7):3257-3262. |
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| 作者姓名： | 程建华 唐翔宇 刘琛 |
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| 作者单位： | 中国科学院、水利部成都山地灾害与环境研究所,山地表生过程与生态调控重点实验室,成都610041;中国科学院大学,北京100049;中国科学院、水利部成都山地灾害与环境研究所,山地表生过程与生态调控重点实验室,成都610041 |
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| 基金项目： | 中国科学院“一三五”规划;国家自然科学基金;国家自然科学基金 |
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| 摘 要： | 鉴于紫色土高垦殖丘陵区土壤中抗生素抗性基因的潜在生态环境风险,本研究采用高通量荧光定量PCR技术,分析了抗生素抗性基因在不同类型畜禽养殖场(养猪场、养鸡场和养牛场)土壤中的丰度和多样性.结果表明,3种养殖场土壤中共检出79种抗生素抗性基因和5种可移动基因元件,其中多重耐药类基因是养殖场土壤中丰度最高的耐药基因.不同养殖场土壤中抗生素抗性基因的丰度和多样性均表现为养鸡场和养猪场样品高于养牛场土壤,且3种养殖场土壤样品中的抗性基因表现出不同的类别分布特征.养殖场土壤中较高的可移动基因元件丰度,及其与抗生素抗性基因丰度显著的相关性(P0. 05)说明,可移动基因元件可能促进了抗生素抗性基因在养殖场土壤中的迁移和扩散.
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| 关 键 词： | 紫色土 畜禽养殖场 抗生素抗性基因(ARGs) 可移动基因元件(MGEs) 高通量荧光定量PCR(HT-QPCR) |
| 收稿时间： | 2018/11/13 0:00:00 |
| 修稿时间： | 2019/1/27 0:00:00 |
Characteristics of Antibiotic Resistance Genes in Various Livestock Feedlot Soils of the Hilly Purple Soil Region |
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| CHENG Jian-hu,TANG Xiang-yu and LIU Chen.Characteristics of Antibiotic Resistance Genes in Various Livestock Feedlot Soils of the Hilly Purple Soil Region[J].Chinese Journal of Environmental Science,2019,40(7):3257-3262. |
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| Authors: | CHENG Jian-hu TANG Xiang-yu and LIU Chen |
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| Institution: | Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China and Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China |
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| Abstract: | Given the potential risk posed by antibiotic resistance genes (ARGs) to the eco-environment in the hilly purple soil region, which has been intensively utilized by human, surface soil samples were collected from feedlots of pig, chicken, and cattle farms and were analyzed for the diversity and abundance of ARGs using high-throughput QPCR. In total, 79 ARGs and 5 mobile genetic elements (MGEs) were detected across all samples. Among these genes, multidrug resistance genes were the most abundant type of ARGs. More abundant and diverse ARGs were observed in feedlot soil samples from pig and chicken farms than those from cattle feedlot soils, and these samples showed different distribution patterns of ARGs. High abundance of MGEs and their significant correlation with ARGs (P<0.05) implied an important role of MGEs in the dissemination of ARGs in livestock feedlot soils. |
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| Keywords: | purple soil livestock feedlot antibiotic resistance genes (ARGs) mobile genetic elements (MGEs) high-throughput QPCR (HT-QPCR) |
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