| HPLC测定川楝子不同采收期及不同部位中川楝素的含量 |
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| 引用本文： | 陈强,周浓,王荣繁.HPLC测定川楝子不同采收期及不同部位中川楝素的含量[J].资源开发与保护,2012(9):777-778,840. |
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| 作者姓名： | 陈强 周浓 王荣繁 |
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| 作者单位： | [1]成都军区机关医院中医科,四川成都610011 [2]重庆三峡学院生命科学与工程学院,重庆万州404000 [3]大理学院药学院,云南大理671000 |
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| 基金项目： | 重庆市高校创新团队建设计划项目（编号：201040） |
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| 摘 要： | 通过测定不同采收期和不同部位川楝子中川楝素的含量,为确定川楝子最佳采收期提供依据。采用高效液相色谱法,色谱柱Agilent Zorbax SB-C18（4.6mm×150mm,5μm）,流动相乙腈—水（28∶72）,流速1.0mL/min,检测波长210nm,柱温35℃。不同采收期川楝子中川楝素以1—2月间采收的含量最高;同一采收期的川楝子果核和果肉中川楝素含量最高,外果皮次之,种子中未检出川楝素。
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| 关 键 词： | 川楝子 川楝素 高效液相色谱法 不同采收期 不同部位 含量测定 |
Determination of Toosendanin in Different Parts of Melia toosendan in Different Seasons by HPLC |
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| Institution: | CHEN Qiang, ZHOU Nong, WANG Rong- fan ( 1. Department of Traditional Chinese Medicine, Military Region Institution Hospital, Chengdu 610011, China; 2. College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, China ; 3. College of Pharmacy, Dali University, Dali 671000, China) |
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| Abstract: | To supply evidence for determining the optimal collecting period for toosendanin in Melia toosendan, HPLC method was established to determine the toosendanin contents from M. toosendan in various collecting periods. The chromatographic conditions were as follows ： Zorbax SB - Cls （4.6mm× 150mm, 5μm） column, acetonitrile - water（28：72, v/v）as mobile phase with a flow rate of 1.0 mL/min, the detection wavelength was at 210 nm and column temperature was at 35℃. Content of toosendanin in the samples collected between January and February was the highest and the contents in core and sarcocarp were higher than in the epicarp, the seeds was not detected toosendanin. It was suggested that M. toosendan should be gathered between January and February. |
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| Keywords: | Melia toosendan toosendanin HPLC different seasons different parts determination |
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