Study of the biouptake of labeled single-walled carbon nanotubes using fluorescence-based method |
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| Authors: | Minghui Yang Seokjoon Kwon Yordan Kostov Avraham Rasooly Govind Rao Upal Ghosh |
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| Affiliation: | (1) Center for Advanced Sensor Technology and Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, 5200 Westland blvd., Baltimore, MD 21227, USA;(2) Department of Civil and Environmental Engineering, University of Maryland, Baltimore County, 5200 Westland blvd., Baltimore, MD 21227, USA;(3) Division of Biology, Office of Science and Engineering, FDA, Silver Spring, MD 20993, USA;(4) National Cancer Institute, Bethesda, MD 20892, USA; |
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| Abstract: | Single-wall carbon nanotubes (CNT) are one of the most attractive engineered nanomaterials due to their unique electrical, mechanical and thermal properties, and potential use in a variety of commercial products. Due to their small size, CNT could become easily airborne and reach the various environmental compartments and eventually the food chain and humans. However, the environmental fate processes and health impacts of CNT are not clear. This study investigated a method for the quantitative measurement of carbon nanotube (CNT) in natural media such soil and benthic organism tissues. Fluorescence dye Nile blue was used for noncovalent labeling of CNT to enable their fluorescence detection. Labeled nanotubes were successfully detected in soil samples as well as in worm tissue. We were also able to detect the presence of labeled carbon nanotubes in worms exposed for 1 week to CNT-laden soil, which indicates CNT may transfer through environmental food web. The method allows for laboratory measurements of CNT mass transfer and partitioning into various environmental systems. |
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