Bioassay-directed chemical analysis of River Elbe surface water including large volume extractions and high performance fractionation |
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Authors: | Reineke N Bester K Hühnerfuss H Jastorff B Weigel S |
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Affiliation: | Institute of Organic Chemistry, University of Hamburg, Germany. |
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Abstract: | A bioassay-directed fractionation and identification (toxicity identification evaluation procedure) was performed on extracts of 10 1 River Elbe water samples. The experimental method included a SDB-1 solid phase extraction followed by RP-HPLC fractionation and subfractionation. Chemical analysis by GC-MS as well as acute toxicity testing using a luminescent bacteria assay were conducted in the respective fractions. Many substances were identified, among which were pesticides and pharmaceuticals, but many compounds remained unknown. |
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