两株有机物降解菌株质粒特性的研究

利用质粒消除法对丙烯酰胺降解菌株B6和四氢呋喃降解菌株THF92株菌株的质粒与性状的相关性进行了初步研究.结果表明,吖啶橙和苯甲酸钠对2株有机物降解菌株的质粒均不能消除,而十二烷基苯硫酸钠(SDS)对2株有机物降解菌株的质粒有部分消除,质粒消除率分别为25%和16.7%.2株有机物降解菌株的质粒消除后菌株的抗性下降,对有机物的耐受性下降,降解能力也下降,推测质粒与菌株生长特性有一定的相关性,可能携带与有机物降解相关的基因.

Studies on the character of plasmids in two organism degrading bacteria strains

The relationship between the plasmid and the character of two strains of acrylamide degradative bactera B6 and tetrahydrofuran degradative bacteria THF9 was studied preliminarily utilizing the plasmid curing technique. Soolium benzoate and acridine orange could not remove all the plasmid in two organism degradative bacteria strains; while SDS could remove partially the plasmid with the rate of curing 25% and 16.7%, respectively. The antibiotic capability of the strains decreased after the plasmid curing, and so did the tolerance capability of the organism decreased and the degrading capability also decreased, conjecturing that the plasmid was related definitely to the growth characters of two bacteria strains, with possibly the relative glue of organism degradation.