Epicuticular wax of large and small white butterflies,<Emphasis Type="Italic"> Pieris brassicae</Emphasis> and<Emphasis Type="Italic"> P. rapae crucivora</Emphasis>: qualitative and quantitative comparison between diapause and non-diapause pupae |
| |
Authors: | Email author" target="_blank">Junichi?KanekoEmail author Chihiro?Katagiri |
| |
Institution: | (1) National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, 062-8555 Sapporo , Japan;(2) Institute of Low Temperature Science, Hokkaido University, 060-0819 Sapporo , Japan |
| |
Abstract: | We compared the quantity and quality of the epicuticular wax of diapause and non-diapause pupae in two closely related Pieris species, P. brassicae and P. rapae crucivora. Main components of their epicuticular wax were identified as hydrocarbons. In P. brassicae, more than 95% of hydrocarbons were saturated regardless of whether the pupae were in diapause or not. In P. rapae crucivora, 93% of hydrocarbons were saturated in non-diapause pupae whereas in diapause pupae 41% were saturated and 59% unsaturated. From measurements of body surface area by nuclear magnetic resonance microimaging, we calculated the average thickness of the wax layer. The thickness in diapause and non-diapause pupae of P. brassicae was 800 and 160 nm, respectively. In P. rapae crucivora, the thickness was 195 nm in diapause and 11 nm in non-diapause. This is the first report to clarify the compositional difference in epicuticular wax between diapause and non-diapause pupae. |
| |
Keywords: | Pieris brassicae Pieris rapae crucivora Diapause Cuticle Hydrocarbons |
本文献已被 PubMed SpringerLink 等数据库收录! |
|