Assessing the estrogenic potency in a Portuguese wastewater treatment plant
using an integrated approach |
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Authors: | Diniz Mário S Maurício Rita Petrovic Mira López de Alda Maria J Amaral Leonor Peres Isabel Barceló Damiá Santana Fernando |
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Institution: | Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia, REQUIMTE, Dep. de Química, Quinta da Torre, 2825 516 Monte da Caparica, Portugal. mesd@fct.unl.pt |
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Abstract: | The estrogenic potency of a wastewater treatment plant (WWTP) was evaluated using chemical and biological analyses, which
showed that after the station treatment processes some of the selected endocrine disruptor compounds (EDCs) were still present in
the treated e uent (e.g., bisphenol A, alkylphenols, estrone). Thus, the most common endocrine EDCs were identified and quantified
and the overall estrogenicity of the treated e uent assessed by integrating the results. Male goldfish (Carassius auratus) were used as
biological indicators in a 28-day experiment. Vitellogenin (Vtg), gonadosomatic and hepatosomatic indices, steroids (17 -estradiol and
11-ketotestosterone) and histopathology were biomarkers used in fish to evaluate WWTP treated e uent estrogenicity, in combination
with instrumental analyses. The results showed a significant increase (P < 0.01) in plasma and liver Vtg, which were significantly
correlated (r = 0.66; P < 0.01). The gonadosmatic index was significantly (P < 0.01) reduced in exposed fish. The steroid analyses
revealed significant elevations in 17 -estradiol and depressed 11-ketotestosterone concentrations. The histological examinations show
changes in exposed fish gonads, such as regressed testes and in some cases (43% to 75%) the development of ovo-testis in fish exposed
to 50% and 100% treated e uent. |
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Keywords: | Carassius auratus endocrine disruptors vitellogenin steroids intersex |
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