| 漆酶/ABTS介体系统对吲哚的降解研究 |
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| 引用本文： | 王玲,陈旺生,吴浩渊,许琴,吴青,任大军.漆酶/ABTS介体系统对吲哚的降解研究[J].环境科学与技术,2012(10):38-41. |
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| 作者姓名： | 王玲 陈旺生 吴浩渊 许琴 吴青 任大军 |
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| 作者单位： | 武汉科技大学资源与环境工程学院 |
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| 基金项目： | 湖北省自然科学基金(2010CDB03502) |
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| 摘 要： | 采用纯漆酶与介体2,2-连氮-双-(3-乙基苯并噻吡咯啉-6-磺酸)(简称ABTS)组成的漆酶/ABTS介体系统对低浓度吲哚进行降解实验。综合考虑,选取降解条件为pH 3、ABTS 0.5 mmol/L,漆酶添加量1 U/mL时,对低浓度吲哚溶液的降解效果最佳,降解率达到80%以上。介体ABTS的加入对降解率的提高发挥了重要作用。体系中决定吲哚降解率高低的重要因素为pH和介体浓度,漆酶添加量影响较小。在HPLC谱图2.4 min和2.7 min出现的新物质可能是氧化吲哚和靛红。
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| 关 键 词： | 漆酶/ABTS介体系统 吲哚 降解 |
Degradation of Indole in Wastewater by Laccase/ABTS Mediator System |
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| WANG Ling,CHEN Wang-sheng,WU Hao-yuan, XU Qin,WU Qing,REN Da-jun.Degradation of Indole in Wastewater by Laccase/ABTS Mediator System[J].Environmental Science and Technology,2012(10):38-41. |
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| Authors: | WANG Ling CHEN Wang-sheng WU Hao-yuan XU Qin WU Qing REN Da-jun |
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| Institution: | (School of Resource and Environmental Engineering,Wuhan University of Science and Technology,Wuhan 430081,China) |
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| Abstract: | Degradation of low concentration indole in wastewater with laccase/ABTS mediator system which consists of laccase and 2,2’-azino-bis(3-ethylbenz-thiazoline-6-sulfonate)(ABTS) was studied.The optimal conditions were obtained as pH 3,ABTS concentration 0.5 mmol/L,laccase addition 1 U/mL.Under the optimal condition,the degradation rate of indole solution reached more than 80%.Additions of ABTS play a crucial role in increasing the rate of degradation.pH and ABTS concentration are important factors in determining the rate of degradation,while laccase addition has little impact.The new substances detected on high performance liquid chromatogram at 2.4 min and 2.7 min may be oxindole and isatin. |
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| Keywords: | laccase/ABTS mediator system indole degradation |
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