| SPE-HPLC法测定水环境中痕量炔雌醇研究 |
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| 引用本文： | 黄敏,陈东辉. SPE-HPLC法测定水环境中痕量炔雌醇研究[J]. 环境科学与技术, 2012, 0(Z1): 23-25 |
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| 作者姓名： | 黄敏 陈东辉 |
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| 作者单位： | 东华大学环境科学与工程学院 |
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| 基金项目： | 上海科委项目(09230500200);教育部博士点基金项目(20090075120007);国家自然科学基金(21007010);中央高校基本科研基金(2011D11309) |
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| 摘 要： | 建立了固相萃取-高效液相色谱法测定水环境中痕量炔雌醇含量的方法。考察了C18小柱的最佳洗脱条件,并且根据出峰时间选择了最佳的色谱条件:流动相为乙腈:水=6:4(V:V),温度为30℃,流速为1.0 mL/min,检测波长为280 nm。线性关系良好,回归方程为y=3.424 8x+0.106,回收率为98%~102%,RSD<3%(n=6)。该方法操作简单,快捷,可以精确地检测到水中雌激素。
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| 关 键 词： | 固相萃取 高效液相色谱 炔雌醇 |
The Study of Determination of Trace EE2 in Water Environment by SPE-HPLC |
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| HUANG Min,CHEN Dong-hui. The Study of Determination of Trace EE2 in Water Environment by SPE-HPLC[J]. Environmental Science and Technology, 2012, 0(Z1): 23-25 |
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| Authors: | HUANG Min CHEN Dong-hui |
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| Affiliation: | (School of Environmental Science and Engineering,Donghua University,Shanghai 201620,China) |
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| Abstract: | SPE-HPLC is used for determination of EE2 in water supply system.The optimal elution condition of C18 column was described.The mobile phase was acetonitrile∶water=(60∶ 40,V/V),the column temperature was 30 ℃,current velocity 1.0 mL/min and detection wavelength was 280 nm on the basis of the peaks appearance time.The calibration curve was linear.The regression equation was y=3.424 8x+0.106.The mean recoveries was 98%~102%,RSD was less than 3%(n=6).The Method is simple,handled easily and accurate for determination of EE2. |
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| Keywords: | solid phase extraction high performance liquid chromatography EE2 |
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