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Holly (Ilex Aquifolium L.) As Zinc and Cadmium Accumulative Indicator in Biogeochemical Prospecting
Authors:P Cellini Legittimo  L Ducceschi  M Martini
Institution:  a Department of Pharmaceutical Sciences, University of Florence, Florence, Italy b Department of Public Health, Epidemiology, and Environmental Analytical Chemistry, University of Florence, Florence, Italy c Department of Earth Sciences, University of Florence, Florence, Italy
Abstract:The results of previous investigations in mineralized and sterile areas of Tuscany (Italy) appeared to point out the special aptitude of holly (Ilex aquifolium L.) in concentrating cadmium and zinc. in the present paper this behaviour has been verified in a mineralized area of Lombardy (Northern Italy).

Samples of leaves, twigs and barks from holly trees were collected, and metal contents (Zn, Cd, and Pb) have been determined by differential pulse anodic stripping voltammetry (DPASV). for comparison, some samples of wood anemone (Anemone nemorosa L.) and soil were also analyzed.

The data obtained confirm a preferential cadmium and zinc accumulation by holly (up to 16 and 1400 μg g-1 dry weight, respectively) also depending on different vegetal organs and vegetative stages of plants. Highest contents of lead have been recovered in the outer portion of bark.
Keywords:Holly  heavy metals  cadmium and zinc accumulative indicator  biogeochemical prospecting
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