氧化亚铁硫杆菌培养条件的筛选及脱硫效果研究

从湖南某煤矿附近的土壤中,分离出能有效脱除燃煤中硫的菌株TFD-9,鉴定为氧化亚铁硫杆菌。通过不同温度、pH值和底物浓度对TFD-9菌生物量的影响,确定了最适宜的培养条件;并试验了该菌株对不同能源物质的利用以及对煤的脱硫能力。实验表明:TFD-9菌的适宜生长条件为温度30℃、Fe2+浓度9g/L、pH值2.0,此时最大生物量达到9.26×107个/mL;菌株对能源物质的利用能力分别为Fe2+100%,S2O32-62%,S少于50%;生物处理20d燃煤总硫脱除率为75.6%,硫化物硫脱除率为82.75%。

Screening of Culture Condition for Thiobacillus Ferrooxidans and Desulfurisation Test

A strain TFD-9 that was able to degrade sulfur in coal was isolated from soil in a coal mine and identified as Thiobacillus ferrooxidans.Through incubating under different conditions,the optimum growth conditions of TFD-9 were obtained.The application of different energy sources as well as desulfurisation of coal was also studied.Results showed that the strain grew best and the max biomass reached to 9.26×10~(7)/mL when proper temperature,pH and substrate concentration were 30℃,pH 2.0 and 9g/L;The energy source was easily applied in Fe~(2+)(100%),S_2O_3~(2-)(62%) and S(less than 50%);The desulfurisation test demonstrated that 75.6% and 82.75% of desulfurisation rate of total sulfur and sulfide could be obtained in the period of 20 days by isolated strain.