| 自制便携式振荡装置/间接竞争酶联免疫吸附法在水库微囊藻毒素-LR快速应急监测中的应用 |
| |
| 引用本文： | 万哲慧,王珅,王奇.自制便携式振荡装置/间接竞争酶联免疫吸附法在水库微囊藻毒素-LR快速应急监测中的应用[J].环境污染与防治,2019,41(6):707-709,742. |
| |
| 作者姓名： | 万哲慧 王珅 王奇 |
| |
| 作者单位： | 温州市环境监测中心站,浙江温州,325000;温州大学生命与环境科学学院,浙江温州,325000 |
| |
| 基金项目： | 浙江省自然科学基金;温州市科技计划;温州市环境保护局生态保护专项资金资助项目 |
| |
| 摘 要： | 应用自制便携式振荡装置,运用间接竞争酶联免疫吸附法(icELISA)建立快速测定水中微囊藻毒素(MC)-LR的现场初筛方法。结果表明:(1)自制便携式振荡装置的检测结果与手工振荡不存在差异,可用自制便携式振荡装置替代手工来进行振荡孵育操作。(2)icELISA建立了快速测定MC-LR的现场监测方法,MC-LR的标准曲线线性关系较好(r=-0.997 0),满足定性定量分析的要求,检出限为0.03μg/L,测定下限为0.12μg/L,空白溶液与实际水样加标回收率为96.2%~113.0%,方法精密度、准确度均较好。
|
| 关 键 词： | 间接竞争酶联免疫吸附法 饮用水源 微囊藻毒素-LR |
Application of self-made portable oscillator/icELISA in rapid emergency monitoring of microcystin-LR in reservoirs |
| |
| WAN Zhehui,WANG Shen,WANG Qi.Application of self-made portable oscillator/icELISA in rapid emergency monitoring of microcystin-LR in reservoirs[J].Environmental Pollution & Control,2019,41(6):707-709,742. |
| |
| Authors: | WAN Zhehui WANG Shen WANG Qi |
| |
| Institution: | (Wenzhou Environmental Monitoring Center,Wenzhou Zhejiang 325000;College of Life and Environmental Science,Wenzhou University,Wenzhou Zhejiang 325000) |
| |
| Abstract: | An icELISA method for the determination of microcystin-LR(MC-LR)in water was developed by using a self-made portable oscillator.The results showed that:(1)there was no difference between the results of selfmade portable oscillator and manual vibration.The self-made portable oscillator could be used to replace manual vibration.(2)A rapid method for on-site monitoring of MC-LR was established by icELISA.A good linear relationship(r=-0.997 0)was obtained,which met the requirements of qualitative and quantitative analysis.Detection limit and low limit of determination of MC-LR were 0.03μg/L and 0.12μg/L separately.With good precision and accuracy,the recovery of blank and actual water sample was 96.2%-113.0%. |
| |
| Keywords: | icELISA drinking-water resource microcystins-LR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
