BDE-47对4种海洋微藻抗氧化酶活性的影响

运用实验生态学和生物化学的方法,研究了不同质量浓度的2,2',4,4'-四溴联苯醚(BDE-47)对海水小球藻Chlorellaautotropica,牟氏角毛藻Chaetoceros muelleri,中肋骨条藻Skeletonema costatum和赤潮异弯藻Heterosigam akashiwo的抗氧化酶活性的影响.结果表明:在实验设定质量浓度范围内(0.1～2.5 μg·L~(-1)),4种微藻的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)均能出现一定的应激活性,以减少BDE-47胁迫对藻细胞自身的危害,但是酶活增加的幅度却有很大不同.与赤潮异弯藻和中肋骨条藻相比,海水小球藻和牟氏角毛藻的SOD和CAT对BDE-47具有较高的敏感性.4种海洋微藻的SOD和CAT对BDE-47的敏感性顺序依次为:海水小球藻>牟氏角毛藻>中肋骨条藻>赤潮异弯藻.因此,海水小球藻的SOD和CAT可以作为指示海洋环境中BDE-47污染水平的生物标志物.

Effects of BDE-47 on the antioxidase activities of four species of marine microalgae

The toxic effects of 2,2',4,4'-tetrabrominated biphenyl ether (BDE-47) at various concentrations on the activities of anti-oxidases in four species of marine microalgae (Chlorella autotropica, Chaetoceros muelleri, Heterosigam akashiwo and Skeletonema costatum) were examined through experimental ecology and biochemica1 methods. The results indicated that both SOD and CAT activities in the four species were enhanced in various degree to alleviate the oxidative damage from BDE-47 in the concentration range of 0.1～2.5μg·L~(-1). Compared with Heterosigam akashiwo and Skeletonema costatum, Chlorella autotropica and Chaetoceros muelleri were more sensitive to BDE-47 concerning SOD and CAT activities. The sensitivity of the four marine microalgae species to BDE-47 was in the order of Chlorella autotropica > Chaetoceros muelleri > Skeletonema costatum > Heterosigam akashiwo. Therefore, SOD and CAT in Chlorella autotropica could be used as the biomarkers to indicate pollution level of BDE-47 in marine environment.