一株辛基酚聚氧乙烯醚降解菌的筛选、鉴定及其降解

采用TX-100为唯一碳源的无机盐培养基进行筛选,经过富集、分离和纯化获得了一株能够耐高浓度TX-100且降解性能较好的降解菌,命名为H1.对细菌H1进行形态学观察、生理生化鉴定和16S rDNA (GenBank Accession No.KC505179)序列相似性分析,初步鉴定为醋酸钙不动杆菌属(Acinetobacter calcoaceticus).通过对菌株H1降解TX-100的产物分析,初步推断TX-100的降解模式为末端氧化,先从EO末端氧化,醚键断裂,形成短链的化合物,同时释放出乙醛酸.质粒检测和消除试验表明,控制菌H1降解TX-100的基因位于质粒上.

Isolation,identification of a octylphenol polyethoxylate degradaing strains and its degradation study

A bacterium strain, designated as H1, was isolated from the inorganic salt medium containing TX-100 as the only carbon source and was capable of degrading high concentrations of TX-100. According to its morphological observation, physiological and biochemical reaction and 16S rDNA (GenBank Accession No.KC505179) sequence analysis, H1strain was identified as Acinetobacter calcoaceticus. The results indicate that the biodegradation process complys with the terminal oxidation model, starting the oxidation of EO end followed the scission of the neighboring ether bond to form the short chains by liberating glyoxylic acid. Plasmid detection and elimination tests show that the gene controlling the degradation of TX-100 is located in plasmid of strain H1.