百喜草茎叶发酵前后甲醇溶提物对离体条件下AM真菌的生长效应

发酵前后百喜草（Paspalumnotatum Fl

EFFECTS OF METHANOLELUATES FRACTIONATED FROM BAHIA GRASS

Effects of methanol (MeOH) eluates fractionated from unfermented an d fermented bahia grass (Paspalum notatum Flügge) (FW)shoot and leave extra cts by flash chromatograph on germination and hyphal growth of AM fungi Gigasp ora margarita Becker & Hall spores in vitro are reported. Unfermented bahi a grass shoots and leaves contained both stimulators and inhibitors for AM fungi growth. Stimulators existed mainly in φ(MeOH)0～25% eluates in which φ (MeOH)25% eluates stimulated hyphal growth of AM fungi most significantly. Inh ibitors basically existed in φ(MeOH)100% eluates. Fermentation effectively decomposed AM fungi hyphal growth inhibitors in fresh bahia grass shoots and lea ves, and preserved stimulators. The optimal mass concentrations of MeOH eluates from fer mented bahia grass shoots and leaves to stimulate hyphal growth of AM fungi were different. In φ(MeOH)0～10% eluates from fermented bahia grass, the treatm ent at ρFW=0.4 g/10 mL in water-agar media most significantly stimul ated the hyphal growth. In φ(MeOH) 25% eluates, treatment at ρFW=0.1 g/10 mL in water-aga r media significantly stimulated hyphal growth. In φ(MeOH) 50% eluates, tre atments at ρFW=1.0 g/10 mL obviously enhanced the hyphal growth. In φ(MeOH)100% eluates, the inhibited phenomena of hyphal growth disappeared i n the treatment at ρFW=0.1 g/10 mL in water-agar media. And with the upgrade of treatment concentrations, the growth inhibitory effect on hyphal gro wth of AM fungi increased. This study strongly suggested that fermented plant sh oots and leaves should be used as agricultural organic fertilizers in order to p erfect the biological character of soils when soil physical and chemical charact ers were mended. Fig 3, Ref 12