Halothiobacillus neapolitanus脱硫性能及限制性因素影响

实验采用气升式反应器考察Halothiobacillus neapolitanus CYJN-1的脱除硫代硫酸盐的效果及其动力学,鉴定不同有机物如醇类、酸类以及重金属离子对其脱硫效果的影响,并比较了反复培养、低温保藏、冷冻干燥、平板保藏和甘油保藏方法的保藏效果。结果表明,通过动力学分析,Halothiobacillus neapolitanus CYJN-1在气升式反应器脱硫过程的最大比生长速率和比消耗速率分别为0.22 h-1和0.23×10-3 g/(cells·h),24 h时转化硫代硫酸钠效率为99%,其中约65%还原态的硫被氧化生成单质硫,产量为0.87 g/L。该菌对甲酸、乙酸等低碳有机物的耐受性较差,对Zn2+、Mn2+和Pb2+离子呈现较强的抗性。中短期保藏过程采用新鲜培养基悬浮4℃保藏效果较佳,保藏1~3个月存活率仍可维持90%左右。

Desulfurization performance of Halothiobacillus neapolitanus and effects of restrictive factors

The performance and kinetics of thiosulfate sulfate-removal by Halothiobacillus neapolitanus CYJN-1 in an airlift reactor were investigated. The effects of different organic substances such as alcohols, acids and heavy metals on the desulfurization efficiency were identified. The preservation efficiencies of repeated culture, cryopreservation, freeze-dried, plating-preservation and glycerol preservation were respectively compared. The results indicated that, with the kinetics analysis, the maximum specific growth rate and consumption ratio of Halothiobacillus neapolitanus CYJN-1 in the airlift reactor were 0.22 h-1 and 0.23×10-3 g/(cells·h), respectively. Conversion efficiency of sodium thiosulfate reached 99% at 24 h. About 65% of reduced sulfur was oxidized to elemental sulfur with the yield of 0.87 g/L. The strain showed less tolerance for low-carbon organic compounds (formic acid and acetic acid) and higher tolerance for Zn2+, Mn2+ and Pb2+. The preservation method of fresh medium suspension at 4℃ was more suitable in the short-term preservation process and the survival rate was maintained still at 90% after being preserved for 1~3 months.